Biochemical profile of Achatina fulica (Mollusca: Gastropoda) after infection by different parasitic loads of Angiostrongylus cantonensis (Nematoda, Metastrongylidae)

• Published in: Journal of invertebrate pathology 2015-01, Vol.124, p.1-5
• Main Authors: Tunholi-Alves, Vinícius Menezes, Tunholi, Victor Menezes, Amaral, Ludimila Santos, Mota, Esther Maria, Júnior, Arnaldo Maldonado, Pinheiro, Jairo, Garcia, Juberlan
• Format: Article
• Language: English
• Subjects: Achatina fulica; Angiostrongylus cantonensis; Animals; Biochemical changes; Gastropoda - metabolism; Gastropoda - parasitology; Hemolymph - metabolism; Histopathological changes; Host-Parasite Interactions; Host–parasite relationship; Nematoda - physiology; Proteins - metabolism; Transaminases - metabolism; Urea - metabolism; Uric Acid - metabolism
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2014.10.001

[Display omitted] •Exposure by A. cantonensis induces proteolysis in A. fulica.•A. Cantonensis increase the activities of aminotransferases in A. fulica.•Exposure by A. cantonensis increases the urea concentration (73.03%) and uric acid (835.94%) in A. fulica.•A. Cantonensis induces histopathological changes in A. fulica. The effect of experimental infection by different parasitic loads of Angiostrongylus cantonensis (Nematode, Metastrongylidae) on the activities of the aminotransferases and concentration of total proteins, uric acid and urea in the hemolymph of Achatina fulica (Mollusca, Gastropoda) were investigated. There was a significant decrease in the concentration of total proteins in the exposed snails to 5000 or more larvae. This change was accompanied by an increase in the concentrations of urea and uric acid in the hemolymph, suggesting a higher rate of deamination of the amino acids. Besides this, variations in the activities of the aminotransferases were also observed, with the highest values recorded in the groups exposed to greater parasite load. These results suggest an increase in the use of total proteins, since there was increased formation of nitrogenous catabolites, in conformity with an increase in the aminotransferase activities. Infection was verified by the fact that L3 larvae recovered from the snails was proportion to the exposure dose of L1 larvae. Histopathological results also indicated presence of an inflammatory cell infiltrate, favoring an increase of both transaminases.