Kainic acid causes redox changes in cerebral cortex extracellular fluid: NMDA receptor activity increases ascorbic acid whereas seizure activity increases uric acid

Kainic acid (KA) causes seizures and extensive brain damage in rats. To study the effects of KA on the redox state in cerebral cortex extracellular fluid (ECF), ascorbic and uric acid concentrations were measured in intracerebral microdialysis samples before and after systemic KA administration (ip)...

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Bibliographic Details
Published in:Neuropharmacology 1998-01, Vol.37 (2), p.149-157
Main Authors: Layton, Matthew E, Samson, Fred E, Pazdernik, Thomas L
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Antioxidants - metabolism
APV
Ascorbic acid
Ascorbic Acid - metabolism
Biological and medical sciences
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
Excitatory Amino Acid Agonists - pharmacology
Extracellular Space - metabolism
Kainic acid
Kainic Acid - pharmacology
Male
Medical sciences
Microdialysis
Midazolam
Midazolam - pharmacology
Midazolam - therapeutic use
Nervous system (semeiology, syndromes)
Nervous system as a whole
Neurology
NMDA
Oxidation-Reduction
Rats
Rats, Wistar
Receptors, N-Methyl-D-Aspartate - drug effects
Receptors, N-Methyl-D-Aspartate - metabolism
Seizures
Seizures - chemically induced
Seizures - metabolism
Uric acid
Uric Acid - metabolism
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Summary:Kainic acid (KA) causes seizures and extensive brain damage in rats. To study the effects of KA on the redox state in cerebral cortex extracellular fluid (ECF), ascorbic and uric acid concentrations were measured in intracerebral microdialysis samples before and after systemic KA administration (ip). During seizures, concentrations of ascorbic and uric acid increased 500 and 100%, respectively. When midazolam was given with KA to prevent seizures, ascorbic acid still increased 400%, but uric acid increased only transiently. When the NMDA receptor antagonist aminophosphonovaleric acid (APV) was included in the microdialysis perfusion media, ascorbic acid levels decreased during baseline perfusion in a concentration-dependent manner. APV then suppressed the KA-induced increase in ascorbic acid levels, without blocking seizure activity. In summary, increased uric acid levels in brain ECF activity after KA administration are related to the induced seizure, but ascorbic acid levels are associated with NMDA receptor activity.
ISSN:0028-3908
1873-7064
DOI:10.1016/S0028-3908(98)00002-1