Generation and characterization of the human immunodeficiency virus type 1 mutants

Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations...

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Bibliographic Details
Published in:Archives of virology 1991-01, Vol.117 (1-2), p.45-58
Main Authors: ADACHI, A, ONO, N, SAKAI, H, OGAWA, K, SHIBATA, R, KIYOMASU, T, MASUIKE, H, UEDA, S
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Western
DNA, Recombinant
DNA, Viral
Fundamental and applied biological sciences. Psychology
Genes, Viral
HIV-1 - genetics
HIV-1 - metabolism
HIV-1 - pathogenicity
HIV-1 - ultrastructure
human immunodeficiency virus 1
Microbiology
Mutagenesis
Mutation - genetics
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Retroviridae Proteins - biosynthesis
RNA-Directed DNA Polymerase - metabolism
Transfection
Virology
Virus Replication - genetics
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Summary:Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, and p15 regions), pol (protease, reverse transcriptase, and endonuclease domains), env (gp120 region), tat, or rev were infectious, whereas vif, vpr, vpu, some of env (gp41) and nef mutants could grow in human CD4+ cells to various degrees. Of the non-infectious mutants, only endonuclease (pol) and gp41 mutants exhibited normal phenotypes with respect to the production of functional reverse transcriptase, the expression of gag, pol, and env proteins, and the generation of progeny virions, when examined in transient assays. All infectious mutants killed the CD4+ cells with the exception of a mutant carrying a defect in the vif gene.
ISSN:0304-8608
1432-8798
DOI:10.1007/bf01310491