Physiological studies on the utilization of oleic acid by Saccharomyces cerevisiae in relation to microbody development

We studied the influence of specific growth conditions on the induction of beta-oxidation enzymes and rate of microbody proliferation in S. cerevisiae by oleic acid. Of all conditions tested, highest enzyme levels and microbody numbers were achieved in glucose-limited continuous cultures, supplement...

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Bibliographic Details
Published in:FEMS microbiology letters 1991-12, Vol.90 (1), p.73-78
Main Authors: Evers, M.E, Hohfeld, J, Kunau, W.H, Harder, W, Veenhuis, M
Format: Article
Language:English
Subjects:
beta-oxidation
Biological and medical sciences
catalase
cell culture
continuous culture
Continuous cultures
enzyme activity
enzymes
epimerase
Fundamental and applied biological sciences. Psychology
genes
growth
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
mutants
Mycology
Oleic acid
organelles
oxidation
Peroxisomes
Peroxisome‐deficient (pas) mutant
Saccharomyces cerevisiae
thiolase
β‐Oxidation
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Summary:We studied the influence of specific growth conditions on the induction of beta-oxidation enzymes and rate of microbody proliferation in S. cerevisiae by oleic acid. Of all conditions tested, highest enzyme levels and microbody numbers were achieved in glucose-limited continuous cultures, supplemented with oleic acids second carbon source. Comparable enzyme levels were observed in identical cultures of peroxisome-deficient (pas) mutants of S. cerevisiae. These experiments showed chemostat cultures on glucose/oleic acid mixtures to be a method of choice for future studies on microbody biogenesis/assembly in constructed, conditional pas mutants.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1991.tb05128.x