Highly efficient genomics-assisted development of a library of introgression lines of Solanum pimpinellifolium

The Solanum pimpinellifolium L. accession TO-937 is resistant to pests due to the presence of type IV glandular trichomes and also has the potential to increase fruit quality traits in tomato cultivars. This accession was selected to develop a genomic library of introgression lines (IL) in the genet...

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Bibliographic Details
Published in:Molecular breeding 2014-12, Vol.34 (4), p.1817-1831
Main Authors: Barrantes, Walter, Fernández-del-Carmen, Asunción, López-Casado, Gloria, González-Sánchez, María Ángeles, Fernández-Muñoz, Rafael, Granell, Antonio, Monforte, Antonio J
Format: Article
Language:English
Subjects:
backcrossing
Biomedical and Life Sciences
Biotechnology
Collection
Cultivars
fruit quality
Gene polymorphism
genetic background
genome
Genomes
genomic libraries
Genomics
Genotyping
introgression
Library collections
Life Sciences
Lycopersicon esculentum
Molecular biology
Nucleotides
Pest resistance
Pests
Plant biology
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Polymorphism
Population number
population size
Recombination
Single-nucleotide polymorphism
Solanum
Solanum pimpinellifolium
Tomatoes
Trichomes
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Summary:The Solanum pimpinellifolium L. accession TO-937 is resistant to pests due to the presence of type IV glandular trichomes and also has the potential to increase fruit quality traits in tomato cultivars. This accession was selected to develop a genomic library of introgression lines (IL) in the genetic background of tomato cultivar “Moneymaker.” In order to increase the accuracy and speed of the IL development process, high-throughput single-nucleotide polymorphism (SNP) genotyping steps were performed in early backcross generations. Five to seven generations were needed to complete the final set of 53 ILs that were characterized with the 8K SNP SOLCAP Infinium array, which demonstrated that the introgressions present in the IL set covered 94 % of the donor genome and that each IL contained an average of 4.25 % (25 Mb) of the donor genome, defining 71 bins of about 10 Mb on average. Additionally, 37 previously undetected, unwanted introgressions were also detected, and most of them very small (
ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-014-0141-0