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Evidence of Antagonistic Regulation of Restart from G1 Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast
Hog1 of Saccharomyces cerevisiae is activated by hyperosmotic stress, and this leads to cell-cycle delay in G 1 , but the mechanism by which cells restart from G 1 delay remains elusive. We found that Whi3, a negative regulator of G 1 cyclin, counteracted Hog1 in the restart from G 1 delay caused by...
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| Published in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (10), p.2002-2007 |
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| Main Authors: | , , , , , , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 2007 |
| container_issue | 10 |
| container_start_page | 2002 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 77 |
| creator | MIZUNUMA, Masaki OGAWA, Takafumi KOYAMA, Tetsuya SHITAMUKAI, Atsunori TSUBAKIYAMA, Ryohei KOMARUYAMA, Tadamasa YAMAGUCHI, Toshinaga KUME, Kazunori HIRATA, Dai |
| description | Hog1 of Saccharomyces cerevisiae is activated by hyperosmotic stress, and this leads to cell-cycle delay in G
1
, but the mechanism by which cells restart from G
1
delay remains elusive. We found that Whi3, a negative regulator of G
1
cyclin, counteracted Hog1 in the restart from G
1
delay caused by osmotic stress. We have found that phosphorylation of Ser-568 in Whi3 by RAS/cAMP-dependent protein kinase (PKA) plays an inhibitory role in Whi3 function. In this study we found that the phosphomimetic Whi3 S568D mutant, like the Δwhi3 strain, slightly suppressed G
1
delay of Δhog1 cells under osmotic stress conditions, whereas the non-phosphorylatable S568A mutation of Whi3 caused prolonged G
1
arrest of Δhog1 cells. These results indicate that Hog1 activity is required for restart from G
1
arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism. |
| doi_str_mv | 10.1271/bbb.130260 |
| format | article |
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1
, but the mechanism by which cells restart from G
1
delay remains elusive. We found that Whi3, a negative regulator of G
1
cyclin, counteracted Hog1 in the restart from G
1
delay caused by osmotic stress. We have found that phosphorylation of Ser-568 in Whi3 by RAS/cAMP-dependent protein kinase (PKA) plays an inhibitory role in Whi3 function. In this study we found that the phosphomimetic Whi3 S568D mutant, like the Δwhi3 strain, slightly suppressed G
1
delay of Δhog1 cells under osmotic stress conditions, whereas the non-phosphorylatable S568A mutation of Whi3 caused prolonged G
1
arrest of Δhog1 cells. These results indicate that Hog1 activity is required for restart from G
1
arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.130260</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>cyclin ; Hog1 ; osmotic stress ; RAS/cAMP-dependent protein kinase ; Saccharomyces cerevisiae ; Whi3</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (10), p.2002-2007</ispartof><rights>2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013</rights><rights>Copyright Japan Science and Technology Agency 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>MIZUNUMA, Masaki</creatorcontrib><creatorcontrib>OGAWA, Takafumi</creatorcontrib><creatorcontrib>KOYAMA, Tetsuya</creatorcontrib><creatorcontrib>SHITAMUKAI, Atsunori</creatorcontrib><creatorcontrib>TSUBAKIYAMA, Ryohei</creatorcontrib><creatorcontrib>KOMARUYAMA, Tadamasa</creatorcontrib><creatorcontrib>YAMAGUCHI, Toshinaga</creatorcontrib><creatorcontrib>KUME, Kazunori</creatorcontrib><creatorcontrib>HIRATA, Dai</creatorcontrib><title>Evidence of Antagonistic Regulation of Restart from G1 Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast</title><title>Bioscience, biotechnology, and biochemistry</title><description>Hog1 of Saccharomyces cerevisiae is activated by hyperosmotic stress, and this leads to cell-cycle delay in G
1
, but the mechanism by which cells restart from G
1
delay remains elusive. We found that Whi3, a negative regulator of G
1
cyclin, counteracted Hog1 in the restart from G
1
delay caused by osmotic stress. We have found that phosphorylation of Ser-568 in Whi3 by RAS/cAMP-dependent protein kinase (PKA) plays an inhibitory role in Whi3 function. In this study we found that the phosphomimetic Whi3 S568D mutant, like the Δwhi3 strain, slightly suppressed G
1
delay of Δhog1 cells under osmotic stress conditions, whereas the non-phosphorylatable S568A mutation of Whi3 caused prolonged G
1
arrest of Δhog1 cells. These results indicate that Hog1 activity is required for restart from G
1
arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism.</description><subject>cyclin</subject><subject>Hog1</subject><subject>osmotic stress</subject><subject>RAS/cAMP-dependent protein kinase</subject><subject>Saccharomyces cerevisiae</subject><subject>Whi3</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpdkUtLxDAUhYMoOD42_oKAGzfV3DRt2uX4HEEQRkVclaRJaoZOokmqzM6fbsu4cnXhnu9czuUgdALkHCiHCynlOeSElmQHzSBnPCtrxnfRjNRQZhUrYB8dxLgiZFwUMEM_N19Waddq7A2euyQ672xMtsVL3Q29SNa7SVrqmERI2AS_xneAr3UvNti6SfjwLmqcPH6Maz9Zn1LQMWK5weld44XvAAun8Ou7zSfL5aCUdR1-0yKmI7RnRB_18d88RC-3N89Xi-zh8e7-av6QWVaUKRuTK-CyJrItqKSQ57IUhqiKaEKE5ka3wKmqhWklVVwX1CjaSiZVyRgrqvwQnW3vfgT_OYzfNGsbW933wmk_xAZKxglhNWEjevoPXfkhuDFdA4xXpKoZwEgVW8o648NafPvQqyaJTe-DCcK1NjY5kGbqpRl7aba95L9W5oB8</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>MIZUNUMA, Masaki</creator><creator>OGAWA, Takafumi</creator><creator>KOYAMA, Tetsuya</creator><creator>SHITAMUKAI, Atsunori</creator><creator>TSUBAKIYAMA, Ryohei</creator><creator>KOMARUYAMA, Tadamasa</creator><creator>YAMAGUCHI, Toshinaga</creator><creator>KUME, Kazunori</creator><creator>HIRATA, Dai</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Oxford University Press</general><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>2013</creationdate><title>Evidence of Antagonistic Regulation of Restart from G1 Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast</title><author>MIZUNUMA, Masaki ; OGAWA, Takafumi ; KOYAMA, Tetsuya ; SHITAMUKAI, Atsunori ; TSUBAKIYAMA, Ryohei ; KOMARUYAMA, Tadamasa ; YAMAGUCHI, Toshinaga ; KUME, Kazunori ; HIRATA, Dai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i456t-451d17b90bc52b2133b6af0d80e00ae7fec172d9afcb2d7e52fd2cb4bd6444583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>cyclin</topic><topic>Hog1</topic><topic>osmotic stress</topic><topic>RAS/cAMP-dependent protein kinase</topic><topic>Saccharomyces cerevisiae</topic><topic>Whi3</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MIZUNUMA, Masaki</creatorcontrib><creatorcontrib>OGAWA, Takafumi</creatorcontrib><creatorcontrib>KOYAMA, Tetsuya</creatorcontrib><creatorcontrib>SHITAMUKAI, Atsunori</creatorcontrib><creatorcontrib>TSUBAKIYAMA, Ryohei</creatorcontrib><creatorcontrib>KOMARUYAMA, Tadamasa</creatorcontrib><creatorcontrib>YAMAGUCHI, Toshinaga</creatorcontrib><creatorcontrib>KUME, Kazunori</creatorcontrib><creatorcontrib>HIRATA, Dai</creatorcontrib><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MIZUNUMA, Masaki</au><au>OGAWA, Takafumi</au><au>KOYAMA, Tetsuya</au><au>SHITAMUKAI, Atsunori</au><au>TSUBAKIYAMA, Ryohei</au><au>KOMARUYAMA, Tadamasa</au><au>YAMAGUCHI, Toshinaga</au><au>KUME, Kazunori</au><au>HIRATA, Dai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence of Antagonistic Regulation of Restart from G1 Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><date>2013</date><risdate>2013</risdate><volume>77</volume><issue>10</issue><spage>2002</spage><epage>2007</epage><pages>2002-2007</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Hog1 of Saccharomyces cerevisiae is activated by hyperosmotic stress, and this leads to cell-cycle delay in G
1
, but the mechanism by which cells restart from G
1
delay remains elusive. We found that Whi3, a negative regulator of G
1
cyclin, counteracted Hog1 in the restart from G
1
delay caused by osmotic stress. We have found that phosphorylation of Ser-568 in Whi3 by RAS/cAMP-dependent protein kinase (PKA) plays an inhibitory role in Whi3 function. In this study we found that the phosphomimetic Whi3 S568D mutant, like the Δwhi3 strain, slightly suppressed G
1
delay of Δhog1 cells under osmotic stress conditions, whereas the non-phosphorylatable S568A mutation of Whi3 caused prolonged G
1
arrest of Δhog1 cells. These results indicate that Hog1 activity is required for restart from G
1
arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><doi>10.1271/bbb.130260</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (10), p.2002-2007 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1647004904 |
| source | Oxford Journals Online; EZB Electronic Journals Library |
| subjects | cyclin Hog1 osmotic stress RAS/cAMP-dependent protein kinase Saccharomyces cerevisiae Whi3 |
| title | Evidence of Antagonistic Regulation of Restart from G1 Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast |
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