Promoter activity analysis and methylation characterization of LTR elements of PERVs in NIH miniature pig

The potential risk of porcine endogenous retrovirus (PERV) transmission is an important issue in xenotransplantation (pig-to-human transplantation). Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promote...

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Bibliographic Details
Published in:Genes & Genetic Systems 2013, Vol.88(2), pp.135-142
Main Authors: Jung, Yi-Deun, Lee, Ja-Rang, Kim, Yun-Ji, Ha, Hong-Seok, Oh, Keon-Bong, Im, Gi-Sun, Choi, Bong-Hwan, Kim, Heui-Soo
Format: Article
Language:English
Subjects:
Animals
Base Sequence
DNA Methylation
Endogenous Retroviruses - genetics
Hep G2 Cells
Humans
long terminal repeat (LTR)
methylation
Molecular Sequence Data
Polymerase Chain Reaction
Porcine endogenous retrovirus
porcine endogenous retrovirus (PERV)
promoter activity
Promoter Regions, Genetic - genetics
Sequence Homology, Nucleic Acid
Swine
Swine, Miniature
Terminal Repeat Sequences - genetics
transcription factor-binding sites
Transcription, Genetic - genetics
United States
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Summary:The potential risk of porcine endogenous retrovirus (PERV) transmission is an important issue in xenotransplantation (pig-to-human transplantation). Long terminal repeats (LTRs) in PERV elements show promoter activity that could affect neighboring functional genes. The methylation status and promoter activities of 3 LTR structures (PERV-LTR1, LTR2, and LTR3 elements) belonging to the PERV-A family were examined using luciferase reporter genes in human liver cell lines (HepG2 and Hep3B). The PERV LTR3 element exhibited hypomethylation and stronger promoter activity than the other LTR elements in human liver cells. We also performed comparative sequences analysis of the PERV LTR elements by using bioinformatics tools. Our findings showed that several transcription factors such as Nkx2-2 and Elk-1 positively influenced the high transcriptional activity of the PERV LTR3 element.
ISSN:1341-7568
1880-5779
DOI:10.1266/ggs.88.135