Effect of dietary isoleucine on the immunity, antioxidant status, tight junctions and microflora in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian)

• Published in: Fish & shellfish immunology 2014-12, Vol.41 (2), p.663-673
• Main Authors: Zhao, Juan, Feng, Lin, Liu, Yang, Jiang, Weidan, Wu, Pei, Jiang, Jun, Zhang, Yongan, Zhou, Xiaoqiu
• Format: Article
• Language: English
• Subjects: Aeromonas; Analysis of Variance; Animals; Antioxidants; Aquaculture - methods; Bacillus; Carps; Cyprinus carpio; Cyprinus carpio var. Jian; Cytokine; Dietary Supplements; DNA Primers - genetics; Dose-Response Relationship, Drug; Escherichia coli; Freshwater; Gene Expression Regulation - drug effects; Intestines - enzymology; Intestines - microbiology; Isoleucine; Isoleucine - administration & dosage; Isoleucine - pharmacology; Lactobacillus; Malondialdehyde - metabolism; Microbiota - drug effects; NF-E2-Related Factor 2 - metabolism; Nuclear factor erythroid 2 (NF-E2)/p45-related factor 2; p38 Mitogen-Activated Protein Kinases - metabolism; Protein Kinase C-delta - metabolism; Real-Time Polymerase Chain Reaction; Tight junction; Tight Junctions - drug effects
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2014.10.002

This study was conducted to investigate the effects of dietary isoleucine (Ile) on the immune response, antioxidant status, tight junctions, and microbial population in the intestine of juvenile Jian carp (Cyprinus carpio var. Jian). A total of 1200 juvenile Jian carp with average initial weight 6.9 ± 0.03 g were fed semi-purified isonitrogenous diets containing 4.2 (unsupplemented control group), 7.0, 9.5, 11.9, 13.9 and 16.9 g Ile kg−1 diet for 60 days. Results indicated that Ile supplementation decreased malondialdehyde (MDA) and protein carbonyl content, and the amounts of Escherichia coli and Aeromonas in the intestine (P