Toll-pathway in tiger shrimp (Penaeus monodon) responds to white spot syndrome virus infection: Evidence through molecular characterisation and expression profiles of MyD88, TRAF6 and TLR genes

• Published in: Fish & shellfish immunology 2014-12, Vol.41 (2), p.441-454
• Main Authors: Deepika, A., Sreedharan, K., Paria, Anutosh, Makesh, M., Rajendran, K.V.
• Format: Article
• Language: English
• Subjects: Animals; Cloning, Molecular; Computational Biology; Decapoda; DNA Primers - genetics; Gene Expression Profiling; Gene Expression Regulation - immunology; Immunity, Innate - genetics; Monodon; Myeloid Differentiation Factor 88 - metabolism; Penaeidae - immunology; Penaeidae - virology; Penaeus monodon; PmMyD88; PmTRAF6; Real-Time Polymerase Chain Reaction; Signal Transduction - genetics; Signal Transduction - immunology; TNF Receptor-Associated Factor 6 - metabolism; Toll-Like Receptors - metabolism; Toll-pathway; White spot syndrome virus; White spot syndrome virus (WSSV); White spot syndrome virus 1 - immunology
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2014.09.026

The Toll-pathway plays key roles in regulating the innate immune response in invertebrates. Myeloid differentiation factor 88 (MyD88) and Tumour necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) are key molecules in this signalling pathway. To investigate the role of Toll-pathway in innate immune response of shrimp, Penaeus monodon, MyD88 (PmMyD88) and TRAF6 (PmTRAF6) were identified and characterised. PmMyD88 cDNA is 1716 bp long with an open reading frame (ORF) of 1449 bp encoding a putative protein of 482 amino acids, with a death domain, a TIR domain and C-terminal extension domain. PmTRAF6 cDNA is 2563 bp long with an ORF of 1785 bp (594 amino acids) with an N-terminal RING-type zinc finger domain, two TRAF-type zinc finger domains, a coiled region and a MATH domain. In healthy shrimp, PmMyD88, PmTRAF6 and PmToll were detected in 15 tissues with the highest expression in midgut, eyestalk and lymphoid organ, respectively. Responses of these genes to WSSV in experimentally-infected P. monodon as well as in cultured haemocytes and also effect of poly I:C on the gene expression in vitro was investigated at six time-points in seven tissues. PmToll showed significant up-regulation at all time-points of infection in six tissues and until 24 h post-infection in vitro. However, poly I:C-induced haemocytes showed up-regulation of the gene until 48 h post-exposure. WSSV caused significant up-regulation of PmMyD88 in most of the tissues tested. The virus challenge as well as poly I:C induction in vitro also resulted in significant up-regulation of the gene. Up-regulated expression of PmTRAF6 was detected in haemocytes and lymphoid organ at late stage of infection. In vitro virus challenge showed significant up-regulation of PmTRAF6 at almost all time-points whereas no significant change in the expression was observed on poly I:C induction. The responses of these key genes, observed in the present study, suggest that Toll-pathway as a whole may play a crucial role in the immune response against viruses in shrimp. •Two key molecules of Toll-pathway – MyD88 and TRAF6 of shrimp, Penaeus monodon were sequenced.•Constitutive expression of the genes and PmTLR was observed in 15 different tissues of healthy shrimp.•White spot syndrome virus (WSSV)-infected shrimp showed increased expression of the genes and PmTLR.•Cultured shrimp haemocytes exposed to WSSV and poly I:C showed increased expression of these genes.•Evidences indicate that Toll-pathway may play a r