Mechanical Wounding of Yeast-Like Conidium Cells of Tremella fuciformis Makes Them Susceptible to Agrobacterium-Mediated Transformation

We developed an efficient protocol that makes possible the practical use of Agrobacterium to transform the yeast-like conidia of Tremella fuciformis. Mechanical wounding of fungal cells prior to co-cultivation with Agrobacterium cells greatly improved transformation efficiency.

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (10), p.2157-2159
Main Authors: SHIN, Dong-Il, PARK, Hee-Sung
Format: Article
Language:English
Subjects:
Agrobacterium
Agrobacterium - genetics
Basidiomycota - cytology
Basidiomycota - genetics
Genetic Engineering - methods
Mechanical Phenomena
mechanical wounding
Spores, Fungal - cytology
transformation
Transformation, Genetic
Tremella fuciformis
yeast-like conidia
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Description
Summary:We developed an efficient protocol that makes possible the practical use of Agrobacterium to transform the yeast-like conidia of Tremella fuciformis. Mechanical wounding of fungal cells prior to co-cultivation with Agrobacterium cells greatly improved transformation efficiency.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.130473