Fission Yeast Leucine-Rich Repeat Protein Lrp1 Is Essential for Cell Morphogenesis as a Component of the Morphogenesis Orb6 Network (MOR)

In eukaryotes, cell morphogenesis is regulated coordinately with the cell cycle. In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (5), p.1086-1091
Main Authors: KUME, Kazunori, KUBOTA, Shunsuke, KOYANO, Takayuki, KANAI, Muneyoshi, MIZUNUMA, Masaki, TODA, Takashi, HIRATA, Dai
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Carrier Proteins - metabolism
Cell Cycle Proteins - metabolism
cell polarity
Cell Proliferation
cell separation
Cytokinesis
Intracellular Signaling Peptides and Proteins - chemistry
Intracellular Signaling Peptides and Proteins - metabolism
leucine-rich repeat
Microtubules - metabolism
Molecular Sequence Data
morphogenesis Orb6 network (MOR)
Protein Serine-Threonine Kinases - metabolism
Protein Transport
Saccharomyces cerevisiae
Schizosaccharomyces - cytology
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe
Schizosaccharomyces pombe Proteins - chemistry
Schizosaccharomyces pombe Proteins - metabolism
Sequence Homology, Amino Acid
yeast
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Summary:In eukaryotes, cell morphogenesis is regulated coordinately with the cell cycle. In fission yeast, the morphogenesis network MOR (morphogenesis Orb6 network) consists of 5 conserved proteins, Pmo25, Nak1, Mor2, Orb6, and Mob2, and is essential for cell polarity control and cell separation following cytokinesis. Here we show that the conserved leucine-rich repeat protein Lrp1 is required for cell morphogenesis as a newly recognized component of MOR. Lrp1 has 4 leucine-rich repeats in its N-terminus and is a homolog of the budding yeast Sog2, which is a component of the RAM network (regulation of Ace2 activity and cellular morphogenesis). Lrp1 was essential for both cell growth and cell morphogenesis as were the other MOR components. Lrp1 was localized to the SPBs (spindle pole bodies, the yeast equivalent of the animal centrosome) throughout the cell cycle and to the medial ring during cytokinesis. Lrp1 interacted with Nak1 and was important for Orb6 kinase activity. Thus Lrp1 proved to function upstream of Orb6 in cell morphogenesis.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.130064