Approach to non-invasive sampling in dengue diagnostics: Exploring virus and NS1 antigen detection in saliva and urine of travelers with dengue

Highlights • 137 serum, urine and saliva samples were collected from 14 travelers. • DENV NS1 and RNA were detected from both urine and saliva. • Prolonged excretion of DENV RNA in urine allows later phase molecular diagnostics. • Concentration of urine did not affect to the detection of DENV RNA in...

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Bibliographic Details
Published in:Journal of clinical virology 2014-11, Vol.61 (3), p.353-358
Main Authors: Korhonen, Essi M, Huhtamo, Eili, Virtala, Anna-Maija K, Kantele, Anu, Vapalahti, Olli
Format: Article
Language:English
Subjects:
Adult
Allergy and Immunology
Antibodies, Viral - blood
Antigens, Viral - analysis
Dengue - diagnosis
Dengue diagnostics
Dengue virus
Dengue Virus - genetics
Dengue Virus - immunology
Dengue Virus - isolation & purification
Enzyme-Linked Immunosorbent Assay
Female
Finland
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Infectious Disease
Male
Molecular Sequence Data
Non-invasive
NS1
Real-Time Polymerase Chain Reaction
Real-time RT-PCR
RNA
RNA, Viral - analysis
RNA, Viral - genetics
Saliva
Saliva - virology
Sensitivity and Specificity
Sequence Analysis, DNA
Serum - virology
Travel
Urine
Urine - virology
Young Adult
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Description
Summary:Highlights • 137 serum, urine and saliva samples were collected from 14 travelers. • DENV NS1 and RNA were detected from both urine and saliva. • Prolonged excretion of DENV RNA in urine allows later phase molecular diagnostics. • Concentration of urine did not affect to the detection of DENV RNA in urine.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2014.08.021