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Approach to non-invasive sampling in dengue diagnostics: Exploring virus and NS1 antigen detection in saliva and urine of travelers with dengue
Highlights • 137 serum, urine and saliva samples were collected from 14 travelers. • DENV NS1 and RNA were detected from both urine and saliva. • Prolonged excretion of DENV RNA in urine allows later phase molecular diagnostics. • Concentration of urine did not affect to the detection of DENV RNA in...
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| Published in: | Journal of clinical virology 2014-11, Vol.61 (3), p.353-358 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c441t-e35424cd66f4a6bd3af54264bd5b7bf2e072e910962a680ff14d826d89132f353 |
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| cites | cdi_FETCH-LOGICAL-c441t-e35424cd66f4a6bd3af54264bd5b7bf2e072e910962a680ff14d826d89132f353 |
| container_end_page | 358 |
| container_issue | 3 |
| container_start_page | 353 |
| container_title | Journal of clinical virology |
| container_volume | 61 |
| creator | Korhonen, Essi M Huhtamo, Eili Virtala, Anna-Maija K Kantele, Anu Vapalahti, Olli |
| description | Highlights • 137 serum, urine and saliva samples were collected from 14 travelers. • DENV NS1 and RNA were detected from both urine and saliva. • Prolonged excretion of DENV RNA in urine allows later phase molecular diagnostics. • Concentration of urine did not affect to the detection of DENV RNA in urine. |
| doi_str_mv | 10.1016/j.jcv.2014.08.021 |
| format | article |
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| ispartof | Journal of clinical virology, 2014-11, Vol.61 (3), p.353-358 |
| issn | 1386-6532 1873-5967 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Adult Allergy and Immunology Antibodies, Viral - blood Antigens, Viral - analysis Dengue - diagnosis Dengue diagnostics Dengue virus Dengue Virus - genetics Dengue Virus - immunology Dengue Virus - isolation & purification Enzyme-Linked Immunosorbent Assay Female Finland Humans Immunoglobulin G - blood Immunoglobulin M - blood Infectious Disease Male Molecular Sequence Data Non-invasive NS1 Real-Time Polymerase Chain Reaction Real-time RT-PCR RNA RNA, Viral - analysis RNA, Viral - genetics Saliva Saliva - virology Sensitivity and Specificity Sequence Analysis, DNA Serum - virology Travel Urine Urine - virology Young Adult |
| title | Approach to non-invasive sampling in dengue diagnostics: Exploring virus and NS1 antigen detection in saliva and urine of travelers with dengue |
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