Effect of selenium supplementation on aflatoxin B1-induced histopathological lesions and apoptosis in bursa of Fabricius in broilers

• Published in: Food and chemical toxicology 2014-12, Vol.74, p.91-97
• Main Authors: Chen, Kejie, Fang, Jing, Peng, Xi, Cui, Hengmin, Chen, Jin, Wang, Fengyuan, Chen, Zhengli, Zuo, Zhicai, Deng, Junliang, Lai, Weimin, Zhou, Yi
• Format: Article
• Language: English
• Subjects: Aflatoxin B 1; Aflatoxin B1 - antagonists & inhibitors; Aflatoxin B1 - toxicity; Animals; Apoptosis; Apoptosis - drug effects; Biological and medical sciences; Broiler; Bursa of Fabricius; Bursa of Fabricius - drug effects; Bursa of Fabricius - pathology; Chickens; Dietary Supplements; Flow Cytometry; Food toxicology; Immunoglobulin; Immunoglobulin A - blood; Immunoglobulin G - blood; Immunoglobulin M - blood; In Situ Nick-End Labeling; Male; Medical sciences; Plant poisons toxicology; Real-Time Polymerase Chain Reaction; Sodium selenite; Sodium Selenite - pharmacology; Toxicology
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2014.09.003

•We investigate the effects of sodium selenite against AFB1 in bursa of Fabricius.•Sodium selenite reduces AFB1-induced histological lesions in bursa of Fabricius.•Sodium selenite decreases AFB1-induced excessive apoptosis of bursa of Fabricius.•Sodium selenite increases the contents of serum immunoglobulin reduced by AFB1.•Sodium selenite reduces the immunotoxicity of AFB1 in bursa of Fabricius. To investigate the effects of sodium selenite against aflatoxin B1 (AFB 1), 200 male Avian broilers, divided into five groups, were fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.3 mg/kg AFB1 + 0.2 mg/kg Se (+Se group I), 0.3 mg/kg AFB1 + 0.4 mg/kg Se (+Se group II) and 0.3 mg/kg AFB1 + 0.6 mg/kg Se (+Se group III), respectively. Compared with the control group, decreased relative weight of bursa of Fabricius and contents of serum immunoglobulin, more vacuoles and debris in the bursal lymphoid follicle, and increased percentage of apoptotic bursal cells were observed in the AFB1 group. Sodium selenite, however, could increase the relative weight of bursa of Fabricius and contents of serum immunoglobulin, and ameliorate histopathological lesions. The percentages of apoptotic bursal cells, through flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method, in the three +Se groups were lower than those in the AFB 1 group. Compared with the AFB 1 group, moreover, the mRNA expressions of Bax and Caspase-3 by qRT-PCR in the three +Se groups were decreased, while the expression of Bcl-2 was increased. The results indicate that sodium selenite in diet can protect chicken from AFB 1-induced impairment of humoral immune function by reducing bursal histopathological lesions and percentages of apoptotic bursal cells.