Transcriptomic Profiling of Human Choriodecidua During Term Labor: Inflammation as a Key Driver of Labor

Problem Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown. Method of study Choriodecidua RNA obtaine...

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Bibliographic Details
Published in:American journal of reproductive immunology (1989) 2015-01, Vol.73 (1), p.36-55
Main Authors: Stephen, Gillian L., Lui, Sylvia, Hamilton, Sarah A., Tower, Clare L., Harris, Lynda K., Stevens, Adam, Jones, Rebecca L.
Format: Article
Language:English
Subjects:
Cell Movement - genetics
Chemokines
choriodecidua
Chorion - physiology
Cytokines - metabolism
Decidua - physiology
Female
Gene Expression Profiling
Humans
Inflammation
Inflammation - genetics
Inflammation - immunology
Inflammation Mediators - metabolism
Labor Onset - immunology
Labor, Obstetric - genetics
Labor, Obstetric - immunology
Leukocytes - immunology
Microarray Analysis
MicroRNAs - genetics
MicroRNAs - metabolism
miRNAs
pathway analysis
Pregnancy
Premature labor
Signal Transduction - genetics
Signal Transduction - immunology
transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Up-Regulation
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Summary:Problem Inflammation is a driver of labor in myometrium and cervix; however, the involvement of decidua is poorly defined. We have reported decidual leukocyte infiltration prior to and during labor; the regulators of these inflammatory processes are unknown. Method of study Choriodecidua RNA obtained after term labor or elective cesarean delivery was applied to Affymetrix GeneChips. Pathway analysis and gene validation were performed. Results Extensive inflammatory activation was identified in choriodecidua following labor, predominantly upregulation of genes regulating leukocyte trafficking and cytokine signalling. Genes governing cell fate, tissue remodelling, and translation were also altered. Upregulation of candidate genes (ICAM1, CXCR4, CD44, TLR4, SOCS3, BCL2A, and IDO) was confirmed. NFκB, STAT1&3, HMGB1, and miRNA‐21, miRNA‐46, miRNA‐141, and miRNA‐200 were predicted upstream regulators. Conclusion This study confirms inflammatory processes are major players in labor events in choriodecidua, as in other gestational tissues. Suppressing uterine inflammation is likely to be critical for arresting premature labor.
ISSN:1046-7408
1600-0897
DOI:10.1111/aji.12328