High Throughput, High Resolution Enzymatic Lithography Process: Effect of Crystallite Size, Moisture, and Enzyme Concentration

By bringing enzymes into contact with predefined regions of a surface, a polymer film can be selectively degraded to form desired patterns that find a variety of applications in biotechnology and electronics. This so-called “enzymatic lithography” is an environmentally friendly process as it does no...

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Bibliographic Details
Published in:Biomacromolecules 2014-12, Vol.15 (12), p.4627-4636
Main Authors: Mao, Zhantong, Ganesh, Manoj, Bucaro, Michael, Smolianski, Igor, Gross, Richard A, Lyons, Alan M
Format: Article
Language:English
Subjects:
Applied sciences
Calorimetry, Differential Scanning
Candida
Caproates - chemistry
Chemical reactions and properties
Degradation
Exact sciences and technology
Fungal Proteins - chemistry
Lactones - chemistry
Lipase - chemistry
Microscopy, Atomic Force
Organic polymers
Physicochemistry of polymers
Polyesters - chemistry
Polymers - chemistry
Surface Properties
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Summary:By bringing enzymes into contact with predefined regions of a surface, a polymer film can be selectively degraded to form desired patterns that find a variety of applications in biotechnology and electronics. This so-called “enzymatic lithography” is an environmentally friendly process as it does not require actinic radiation or synthetic chemicals to develop the patterns. A significant challenge to using enzymatic lithography has been the need to restrict the mobility of the enzyme in order to maintain control of feature sizes. Previous approaches have resulted in low throughput and were limited to polymer films only a few nanometers thick. In this paper, we demonstrate an enzymatic lithography system based on Candida antartica lipase B (CALB) and poly(ε-caprolactone) (PCL) that can resolve fine-scale features, (
ISSN:1525-7797
1526-4602
DOI:10.1021/bm501475n