Overexpression of archaeal proteins in Escherichia coli

Six archaeal proteins containing a high number of Escherichia coli rare codons in their genes were not well expressed in E. coli. These genes showed a five to twenty-fold increase in production when expressed in the presence of a plasmid harboring and expressing the argU and ileX genes encoding rare...

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Bibliographic Details
Published in:Biotechnology letters 1998-03, Vol.20 (3), p.207-210
Main Authors: KIM, R, SANDLER, S. J, GOLDMAN, S, YOKOTA, H, CLARK, A. J, KIM, S.-H
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biotechnology
E coli
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Modification of gene expression level
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Summary:Six archaeal proteins containing a high number of Escherichia coli rare codons in their genes were not well expressed in E. coli. These genes showed a five to twenty-fold increase in production when expressed in the presence of a plasmid harboring and expressing the argU and ileX genes encoding rare tRNAs (tRNA arg(de)AGA/AGG and tRNA ile(de)AUA. © Rapid Science Ltd. 1998[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/a:1005305330517