Sex Identification in the Egyptian Vulture by Flow Cytometry and Cytogenetics

The genome size and sex of the Egyptian Vulture (Neophron percnopterus), a sexually monomorphic bird, were identified from blood samples by the use of flow cytometry (FCM). This technique allowed evaluation of the slightly higher amount of DNA content in the homogametic male compared to that of the...

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Bibliographic Details
Published in:The Condor (Los Angeles, Calif.) Calif.), 1997-08, Vol.99 (3), p.829-832
Main Authors: Cavallo, Delia, De Vita, Raffaele, Eleuteri, Patrizia, Robert H. R. Belterman, Dell'Omo, Giacomo
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birds
Blood
Cellular biology
Chickens
Cytogenetics
Cytometry
Deoxyribonucleic acid
DNA
Erythrocytes
Female animals
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Genome size
Lymphocytes
Male animals
Ornithology
Sex chromosomes
Sexes
Short Communications
Vertebrata
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Summary:The genome size and sex of the Egyptian Vulture (Neophron percnopterus), a sexually monomorphic bird, were identified from blood samples by the use of flow cytometry (FCM). This technique allowed evaluation of the slightly higher amount of DNA content in the homogametic male compared to that of the heterogametic female. A karyotypic analysis also was performed in order to confirm the FCM results. Sex identification by both FCM and cytogenetic analyses was concordant in all cases. The average DNA content was estimated to be 5.6% higher in males than in females.
ISSN:0010-5422
1938-5129
2732-4621
DOI:10.2307/1370498