Direct antigen detection in Langmuir—Blodgett films of immunoglobulin G modified with coproporphyrin-I

A direct optical method based on the interaction of antigen with a thin film of immunoglobulin G (IgG) modified with a fluorescent label was developed for the fluorimetric determination of antigen—antibody binding. The analytical system uses a Langmuir—Blodgett film of coproporphyrin I-modified IgG...

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Bibliographic Details
Published in:Analytica chimica acta 1992-08, Vol.265 (1), p.21-26
Main Authors: Turko, Illarion V., Lepesheva, Galina I., Chashchin, Vadim L.
Format: Article
Language:English
Subjects:
Antigen—antibody binding
Biological and medical sciences
Biosensors
Biotechnology
Coproporphyrin-I
Fluorimetry
Fundamental and applied biological sciences. Psychology
Immunoassay
Immunoglobulin G
Langmuir—Blodgett films
Methods. Procedures. Technologies
Various methods and equipments
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Summary:A direct optical method based on the interaction of antigen with a thin film of immunoglobulin G (IgG) modified with a fluorescent label was developed for the fluorimetric determination of antigen—antibody binding. The analytical system uses a Langmuir—Blodgett film of coproporphyrin I-modified IgG which binds antigen and changes its fluorescent properties. The enhanced fluorescence is linear with respect to increasing concentration of antigen over a broad range. The main advantage of the proposed method is the possibility of developing a direct immunoassay based on evanescent excited fibre-optics.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(92)85150-5