Xenobiotic Interaction with and Alteration of Channel Catfish Estrogen Receptor

• Published in: Toxicology and applied pharmacology 1997-12, Vol.147 (2), p.381-390
• Main Authors: Nimrod, Alison C., Benson, William H.
• Format: Article
• Language: English
• Subjects: Air; Animals; Biological and medical sciences; Environmental pollutants toxicology; Estradiol - pharmacology; Ictaluridae; Insecticides - pharmacology; Liver - drug effects; Liver - metabolism; Medical sciences; Phenols - pharmacology; Receptors, Estrogen - agonists; Receptors, Estrogen - metabolism; Toxicology; Xenobiotics - pharmacology
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1006/taap.1997.8296

In teleostean in vivostudies, the vitellogenin response to environmental estrogens is not completely predicted by mammalian literature. One possible explanation for differences is heterogeneity of the estrogen receptor (ER) structure between species. Therefore, ER from channel catfish ( Ictalurus punctatus) hepatic tissue was characterized by binding affinity for several compounds. Affinity was indirectly measured as potency of the chemical for inhibiting binding of radiolabeled estradiol (E2) to specific binding sites. The order of potency among therapeutic chemicals was ethinylestradiol > unlabeled E2 = diethylstilbestrol > mestranol > tamoxifen ⪢ testosterone. Unlabeled E2 had an IC50 of 2.2 n m. Several environmentally relevant chemicals were evaluated in a similar manner and the order of potency established was the o-demethylated metabolite of methoxychlor (MXC) > nonylphenol (NP) > chlordecone > MXC > o,p′-DDT > o,p′-DDE > β-hexachlorocyclohexane. Demethylated MXC had an IC50 1000-fold greater than that of E2. Of the most potent inhibitors, NP appeared to be a competitive inhibitor for the same binding site as E2, while o-demethylated MXC had a more complex interaction with the receptor protein. ER from nonvitellogenic females was determined to have a K d value of 1.0 to 1.3 n m. Because E2 has been reported to up-regulate teleostean ER, the hepatic ER population following in vivoxenobiotic exposure was assessed. NP significantly increased ER per milligram hepatic protein almost to the same extent as E2, but did not increase K d to the same extent as E2.