Determination of ligninase activity in P. chrysosporium pellets with diffuse reflectance spectrophotometry

Diffuse reflectance spectrophotometry was applied for measuring ligninase activity in pellets of Phanerochaete chrysosporium. Enhanced ligninase activity in pellets and in growth medium were detected in cultures supplemented with oleic acid emulsified with Tween 80, Tween 80, hydrophilic (alcoholic)...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1993, Vol.38 (4), p.570-573
Main Authors: Lestan, D, Cernilec, M, Perdih, A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
enzyme activity
Fundamental and applied biological sciences. Psychology
ligninolytic microorganisms
Miscellaneous
Mission oriented research
Phanerochaete chrysosporium
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Summary:Diffuse reflectance spectrophotometry was applied for measuring ligninase activity in pellets of Phanerochaete chrysosporium. Enhanced ligninase activity in pellets and in growth medium were detected in cultures supplemented with oleic acid emulsified with Tween 80, Tween 80, hydrophilic (alcoholic) residue of Tween 80 hydrolysate, Tween 20, and (15OE)C18:1. In cultures with low extracellular ligninase activity, low activity in pellets was also observed. Our results indicate that the stimulatory effect of the tested surfactants cannot be contributed solely to promotion of ligninases through the cell membrane.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00242957