Differing levels of Mhc class II β chain expression in a range of tissues from vaccinated and non-vaccinated Atlantic salmon (Salmo salarL.)

The expression of major histocompatibility complex class II β chain (Mhc class II β chain) was investigated in the tissues from vaccinated and non-vaccinated Atlantic salmon (Salmo salarL.). A reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used to detect messenger ribonucleic...

Full description

Saved in:
Bibliographic Details
Published in:Fish & shellfish immunology 1998-04, Vol.8 (3), p.183-196
Main Authors: KOPPANG, E.O., LUNDIN, M., PRESS, C.McL, RØNNINGEN, K., LIE, Ø.
Format: Article
Language:English
Subjects:
Atlantic salmon
expression
major histocompatibility complex class II
Mhc class II
Salmo salar
Salmo salarL
tissues
vaccine response
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The expression of major histocompatibility complex class II β chain (Mhc class II β chain) was investigated in the tissues from vaccinated and non-vaccinated Atlantic salmon (Salmo salarL.). A reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used to detect messenger ribonucleic acid (mRNA) in brain, skeletal muscle, heart, liver, foregut, head kidney, spleen, hindgut and gills. A 313 nucleotide RT-PCR product was produced and sequence analysis demonstrated that the amplified product was from the βchain of Mhc-Sasa class II. After 20 cycles of PCR, expression of Mhc class II β chain was only detected in the foregut, spleen, hindgut and gills of non-vaccinated fish. The same number of cycles of PCR detected expression in a broader range of tissues from vaccinated fish, including heart, liver, foregut, head kidney, spleen, hindgut and gills. There was low or negligible expression in brain and skeletal muscle. In the tissues from both groups there was variation in the level of expression of Mhc class II β chain between fish. Computer-assisted morphometric analysis of agarose gels scanned on a UV-light table allowed a semi-quantitative estimate of the level of Mhc class II β chain expression in the different tissues from the two groups, each of which contained five fish. Of those tissues showing expression in both vaccinated and non-vaccinated fish, there were significant increases in expression in the spleen and gills but not the foregut and hindgut of vaccinated fish (P
ISSN:1050-4648
1095-9947
DOI:10.1006/fsim.1997.0136