Fluorescent sensors using DNA-functionalized graphene oxide

In the past few years, graphene oxide (GO) has emerged as a unique platform for developing DNA-based biosensors, given the DNA adsorption and fluorescence-quenching properties of GO. Adsorbed DNA probes can be desorbed from the GO surface in the presence of target analytes, producing a fluorescence...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2014-11, Vol.406 (27), p.6885-6902
Main Authors: Liu, Zhenbao, Liu, Biwu, Ding, Jinsong, Liu, Juewen
Format: Article
Language:English
Subjects:
Adsorption
Analysis
Analytical Chemistry
Aptamers
Biochemistry
Biosensors
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Covalence
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA probes
Environmental monitoring
Fluorescence
Fluorescent Dyes - chemistry
Food Science
Graphene
Graphene in Analytics
Graphite - chemistry
Laboratory Medicine
Monitoring/Environmental Analysis
Oxides
Oxides - chemistry
Probes
Quenching
Review
Sensors
Strategy
Surface chemistry
Technology application
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Summary:In the past few years, graphene oxide (GO) has emerged as a unique platform for developing DNA-based biosensors, given the DNA adsorption and fluorescence-quenching properties of GO. Adsorbed DNA probes can be desorbed from the GO surface in the presence of target analytes, producing a fluorescence signal. In addition to this initial design, many other strategies have been reported, including the use of aptamers, molecular beacons, and DNAzymes as probes, label-free detection, utilization of the intrinsic fluorescence of GO, and the application of covalently linked DNA probes. The potential applications of DNA-functionalized GO range from environmental monitoring and cell imaging to biomedical diagnosis. In this review, we first summarize the fundamental surface interactions between DNA and GO and the related fluorescence-quenching mechanism. Following that, the various sensor design strategies are critically compared. Problems that must be overcome before this technology can reach its full potential are described, and a few future directions are also discussed.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-014-7888-3