Preparation of poly(vinylphenylboronic acid) chain grafted poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads for the selective enrichment of glycoprotein

Surface‐initiated atom transfer radical polymerization was successfully used to prepare 4‐vinylphenylboronic acid functionalized poly(glycidylmethacrylate‐co‐ethylenedimethacrylate) beads for the selective enrichment of glycoprotein from complex biological samples in this study. The modified bead su...

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Bibliographic Details
Published in:Journal of separation science 2015-01, Vol.38 (1), p.81-86
Main Authors: Zhang, Jing, Ni, Yan-li, Zheng, Xiao-ling
Format: Article
Language:English
Subjects:
Acids
Adsorption
alpha-Fetoproteins - chemistry
alpha-Fetoproteins - isolation & purification
Analytic Sample Preparation Methods - methods
Animals
Atom transfer radical polymerization
Beads
Biological
Boronic acid
Boronic Acids - chemistry
Brushes
Chickens
Egg White - chemistry
Enrichment
Fourier transforms
Glycoprotein
Glycoproteins - chemistry
Glycoproteins - isolation & purification
Grafting
Humans
Methylmethacrylates - chemistry
Polymerization
Polymers - chemical synthesis
Polymers - chemistry
Surface chemistry
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Summary:Surface‐initiated atom transfer radical polymerization was successfully used to prepare 4‐vinylphenylboronic acid functionalized poly(glycidylmethacrylate‐co‐ethylenedimethacrylate) beads for the selective enrichment of glycoprotein from complex biological samples in this study. The modified bead surfaces were characterized using Fourier transform infrared spectroscopy and X‐ray photoelectron spectroscopy. The sorption behaviors, including adsorption isotherms, incubation time, and pH effect, were investigated. The results demonstrated that the boronated beads have a high affinity for glycoprotein, which is due to the well‐defined boronic acid brushes on the beads surfaces. Furthermore, the polyvinylphenylboronic acid grafted poly(glycidylmethacrylate‐co‐ethylenedimethacrylate) beads were used to efficiently enrich and purify glycoprotein from real egg white samples and α‐fetoprotein from human serum samples. The mass spectrometry results demonstrated that the polyvinylphenylboronic acid grafted poly(glycidylmethacrylate‐co‐ethylenedimethacrylate) beads are a suitable material for the enrichment of glycosylated protein from complex biological samples.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201400907