Rice transgene flow: its patterns, model and risk management

• Published in: Plant biotechnology journal 2014-12, Vol.12 (9), p.1259-1270
• Main Authors: Jia, Shirong, Yuan, Qianhua, Pei, Xinwu, Wang, Feng, Hu, Ning, Yao, Kemin, Wang, Zhixing
• Format: Article
• Language: English
• Subjects: Agriculture; China; Commercialization; common wild rice (O. rufipogon); Crops; Cultivars; Environmental risk; Flowering; Flowers & plants; Gene Flow; gene flow model; Genes; Genetically altered foods; Herbicides; Hybrids; Mathematical models; Models, Genetic; Oryza - genetics; Oryza sativa; Plants, Genetically Modified; Pollen; Rice; rice (Oryza sativa L.); Risk Assessment; risk assessment and management; Risk management; Seeds; Thresholds; transgene flow; Transgenes; Transgenes - genetics; Transgenic; weedy rice; Wind speed
• ISSN: 1467-7644; 1467-7652
• DOI: 10.1111/pbi.12306

Summary Progress has been made in a 12 year's systemic study on the rice transgene flow including (i) with experiments conducted at multiple locations and years using up to 21 pollen recipients, we have elucidated the patterns of transgene flow to different types of rice. The frequency to male sterile lines is 101 and 103 higher than that to O. rufipogon and rice cultivars. Wind speed and direction are the key meteorological factors affecting rice transgene flow. (ii) A regional applicable rice gene flow model is established and used to predict the maximum threshold distances (MTDs) of gene flow during 30 years in 993 major rice producing counties of southern China. The MTD0.1% for rice cultivars is basically ≤5 m in the whole region, despite climate differs significantly at diverse locations and years. This figure is particularly valuable for the commercialization and regulation of transgenic rice. (iii) The long‐term fate of transgene integrated into common wild rice was investigated. Results demonstrated that the F1 hybrids of transgenic rice/O. rufipogon gradually disappeared within 3–5 years, and the Bt or bar gene was not detectable in the mixed population, suggesting the O. rufipogon may possess a strong mechanism of exclusiveness for self‐protection. (iv) The flowering time isolation and a 2‐m‐high cloth‐screen protection were proved to be effective in reducing transgene flow. We have proposed to use a principle of classification and threshold management for different types of rice.