Molecularly imprinted polymers with synthetic dummy templates for the preparation of capsaicin and dihydrocapsaicin from chili peppers

• Published in: Journal of separation science 2015-01, Vol.38 (1), p.100-107
• Main Authors: Ma, Xiuli, Ji, Wenhua, Chen, Lingxiao, Wang, Xiao, Liu, Jianhua, Wang, Xueyong
• Format: Article
• Language: English
• Subjects: Accessibility; Adsorption; Capsaicin - analogs & derivatives; Capsaicin - chemistry; Capsaicin - isolation & purification; Capsaicinoids; Capsicum - chemistry; Chili peppers; Dummies; Dummy template; Enrichment; Imprinted polymers; Isotherms; Mathematical analysis; Molecular imprinted polymers; Molecular Imprinting; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Polymers; Polymers - chemical synthesis; Polymers - chemistry; Solid Phase Extraction - instrumentation; Solid Phase Extraction - methods; Solid-phase extraction; Yeast
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.201400911

In this work, dummy molecularly imprinted polymers with high selectivity and affinity to capsaicin and dihydrocapsaicin are designed using N‐vanillylnonanamide as a dummy template. The performance of dummy molecularly imprinted polymers and nonimprinted polymers was evaluated using adsorption isotherms, adsorption kinetics, and selective recognition capacity. Dummy molecularly imprinted polymers were found to exhibit good site accessibility, taking just 20 min to achieve adsorption equilibrium; they were also highly selective toward capsaicin and dihydrocapsaicin. We successfully used dummy molecularly imprinted polymers as a specific sorbent for selectively enriching capsaicin and dihydrocapsaicin from chili pepper samples. In a scaled‐up experiment, the selective recovery of capsaicinoids was calculated to be 77.8% using solid‐phase extraction. To the best of our knowledge, this is the first example of the use of N‐vanillylnonanamide as a dummy template in molecularly imprinted polymers to simultaneously enrich capsaicin and dihydrocapsaicin.