A new type of glucose biosensor based on surface acoustic wave resonator using Mn-doped ZnO multilayer structure

• Published in: Biosensors & bioelectronics 2013-11, Vol.49, p.512-518
• Main Authors: Luo, Jingting, Luo, Pingxiang, Xie, Min, Du, Ke, Zhao, Bixia, Pan, Feng, Fan, Ping, Zeng, Fei, Zhang, Dongping, Zheng, Zhuanghao, Liang, Guangxing
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensor; Biosensors; Biotechnology; Blood Glucose - analysis; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Equipment Design; Fundamental and applied biological sciences. Psychology; General aspects; Glucose; Glucose Oxidase - chemistry; Glucose Oxidase - metabolism; Glucose oxidase functional immobilization; Humans; Immobilization techniques; Manganese; Manganese - chemistry; Methods. Procedures. Technologies; Monitoring; Multilayers; Sensitivity and Specificity; Sound; Sugars; Surface acoustic wave (SAW); Surface acoustic waves; Various methods and equipments; Zinc oxide; Zinc Oxide - chemistry; ZnO
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2013.05.021

This work reports a high-performance Mn-doped ZnO multilayer structure Love mode surface acoustic wave (SAW) biosensor for the detection of blood sugar. The biosensor was functionalized via immobilizing glucose oxidase onto a pH-sensitive polymer which was attached on Mn-doped ZnO biosensor. The fabricated SAW glucose biosensor is highly sensitive, accurate and fast with good anti-interference. The sensitivity of the SAW glucose biosensor is 7.184MHz/mM and the accuracy is 6.96×10−3mM, which is sensitive and accurate enough for glucose monitoring. A good degree of reversibility and stability of the glucose sensor is also demonstrated, which keeps a constant differential frequency shift up to 32 days. Concerning the time response to human serum, the glucose sensor shows a value of 4.6±0.4min when increasing glucose concentrations and 7.1±0.6min when decreasing, which is less than 10min and reach the fast response requirement for medical applications. The Mn-doped ZnO Love mode SAW biosensor can be fully integrated with CMOS Si chips and developed as a portable, passive and wireless real time detection system for blood sugar monitoring in human serum. •SAW biosensor was used to monitor blood sugar for the first time.•High performance SAW biosensor was fabricated on Mn-doped ZnO multilayer structure.•SAW glucose biosensor system was sensitive and accurate with good reversibility and stability.