Adsorption induced denaturation: application to denaturation of soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) in soymilk

• Published in: Biotechnology letters 2015-01, Vol.37 (1), p.147-151
• Main Authors: Jasti, Lakshmi Swarnalatha, Lavanya, Kuna, Fadnavis, Nitin W
• Format: Article
• Language: English
• Subjects: Acetic acid; Adsorption; Amines; Applied Microbiology; Binding; binding capacity; Biochemistry; Biomedical and Life Sciences; Biotechnology; composite polymers; Copolymers; Crosslinking; Denaturation; Density; Epoxy Compounds - chemistry; Food science; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; hydrophobicity; Life Sciences; Lipoxygenase; Lipoxygenase - chemistry; Lipoxygenase - isolation & purification; Lipoxygenase - metabolism; Liquid oxygen; Methacrylates - chemistry; Microbiology; Original Research Paper; Polymers; Protein Denaturation; proteins; Soy Milk - chemistry; Soy products; Soybean Proteins - chemistry; Soybean Proteins - isolation & purification; Soybean Proteins - metabolism; Soybeans; soymilk; temperature; Trypsin inhibitors; Trypsin Inhibitors - chemistry; Trypsin Inhibitors - isolation & purification; Trypsin Inhibitors - metabolism
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-014-1659-2

Allyl glycidyl ether-ethylene glycol dimethacrylate copolymer with 25 % crosslink density has been functionalized with various amines. The polymer bearing a dibenzyl amino function efficiently removed the soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) from soymilk. The polymer binds SBTI and LOX efficiently (25–30 mg/g) through a combination of hydrophobic and ionic interactions and denatures them at room temperature by simple contact for 1 h. The bound proteins can be easily recovered almost quantitatively by elution with 1 % (v/v) acetic acid, and the polymer can be recycled at least 10 times without significant changes in binding capacity.