In situ SERS probing of nano-silver coated individual yeast cells

For understanding cells functionalities and their communications, there is a need for highly sensitive cell analysis platforms capable of assessing non-specific chemicals on the surface and in the vicinity of cells. We report a microfluidic system integrating dielectrophoresis and surface enhanced R...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2013-11, Vol.49, p.536-541
Main Authors: Chrimes, Adam F., Khoshmanesh, Khashayar, Tang, Shi-Yang, Wood, Bayden R., Stoddart, Paul R., Collins, Sean S.E., Mitchell, Arnan, Kalantar-zadeh, Kourosh
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biosensors
Biotechnology
Coating
Dielectrophoresis
Equipment Design
Fundamental and applied biological sciences. Psychology
Metal Nanoparticles - chemistry
Metal Nanoparticles - ultrastructure
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Monitoring
Nanostructure
Principal Component Analysis
Raman scattering
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - metabolism
SERS
Silver
Silver - chemistry
Spectrum Analysis, Raman - instrumentation
Trapping
Yeast
Yeast cell
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Summary:For understanding cells functionalities and their communications, there is a need for highly sensitive cell analysis platforms capable of assessing non-specific chemicals on the surface and in the vicinity of cells. We report a microfluidic system integrating dielectrophoresis and surface enhanced Raman scattering (SERS) for the trapping and real time monitoring of cell functions in isolated and grouped cell clusters. Yeast cells are coated with silver nanoparticles to enable highly sensitive SERS analysis. The SERS responses of cells are examined under various conditions: live vs. dead and isolated vs. grouped. This work illustrates the feasibility of the system for in situ cell monitoring and analysis of secreted chemicals during their growth, metabolism, proliferation and apoptosis. •Yeast cells are coated with Raman enhancing silver nanoparticles.•Dielectrophoresis is used to trap cells in configurable patterns for Raman analysis.•Raman signatures of dead and live yeast compared using principal component analysis.•Raman signatures from isolated cells are compared to that from cells in groups.•Principal component analysis identified glycine as a possible proliferation marker.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2013.05.053