Rapid differentiation of in vitro cellular responses to toxic chemicals by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Changes in protein expression as a cellular response to chemical exposure have been well established. Current methods for monitoring cellular responses usually require the use of specific reagents and/or labor‐intensive procedures. The present study demonstrates the concept of using mass spectral pa...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 2015-01, Vol.34 (1), p.161-166
Main Authors: Chiu, Norman H.L., Jia, Zhenquan, Diaz, Reynaldo, Wright, Petra
Format: Article
Language:English
Subjects:
Aflatoxin B1 - toxicity
Analytical chemistry
Biotechnology
Cell culture
Cell Line
Cellular
Cellular biology
Chemicals
Cytotoxicity
Desorption
Hazard/risk assessment
Humans
Hydrogen Peroxide - toxicity
Ionization
Lasers
Mass spectrometry
Monitoring methods
Principal Component Analysis
Protein expression
Proteome - metabolism
Reagents
Risk assessment
Spectra
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Toxicology
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Summary:Changes in protein expression as a cellular response to chemical exposure have been well established. Current methods for monitoring cellular responses usually require the use of specific reagents and/or labor‐intensive procedures. The present study demonstrates the concept of using mass spectral pattern to distinguish different cellular responses. The concept is based on the ability to acquire a unique mass spectral pattern directly from a specific cell culture by using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). The results demonstrate that distinguishable and reproducible spectral patterns can be obtained from different cellular responses. Environ Toxicol Chem 2015;34:161–166. © 2014 SETAC
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.2774