Cells–nano interactions and molecular toxicity after delayed hypersensitivity, in Guinea pigs on exposure to hydroxyapatite nanoparticles

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2013-12, Vol.112, p.204-212
• Main Authors: Geetha, C.S., Remya, N.S., Leji, K.B., Syama, S., Reshma, S.C., Sreekanth, P.J., Varma, H.K., Mohanan, P.V.
• Format: Article
• Language: English
• Subjects: 8-OHdG; Animals; Antioxidant enzymes; Biocompatibility; blood composition; brain; Cell Line; Cell Survival - drug effects; colloids; cytotoxicity; Damage; Delayed hypersensitivity; DNA Damage; Durapatite - chemistry; Durapatite - immunology; Durapatite - toxicity; Exposure; Guinea Pigs; HANP; Hydroxyapatite; Hypersensitivity, Delayed - chemically induced; in vitro studies; in vivo studies; Lipid Peroxidation - drug effects; Liver; Liver - drug effects; Liver - metabolism; LPO; Mice; Microscopy, Electron, Scanning; nanoparticles; Nanoparticles - chemistry; Nanoparticles - toxicity; Nanoparticles - ultrastructure; Nanotechnology; oxidative stress; Oxidative Stress - drug effects; Rats; Rats, Wistar; Skin - drug effects; Skin - immunology; Surgical implants; Toxicity
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2013.07.058

•HANP was synthesized by chemical method.•Characterized of HANP by TEM, SEM, FT-IR, XRD.•The HANPs were biocompatible with more than 80% viable.•Non-skin irritant after skin sensitization in G. pigs.•Not altered the antioxidant potential in vitro and in vivo. The aim of the study was to evaluate the cells–nanoparticle interactions and molecular toxicity after delayed hypersensitivity in Guinea pigs, exposed to hydroxyapatite nanoparticles (HANP). The study focuses on synthesizing and characterizing HANPs and gaining an insight into the cytotoxicity, molecular toxicity, hypersensitivity and oxidative stress caused by them in vitro and in vivo. HANP was synthesized by chemical method and characterized by standard methods. Cytotoxicity was assessed on L929 cells by MTT assay and in vitro studies were carried out on rat liver homogenate. In vivo study was carried out by topical exposure of Guinea pigs with HANP, repeatedly, and evaluating the skin sensitization potential, blood parameters, oxidative stress in liver and brain and DNA damage (8-hydroxyl-2-deoxyguanosine: 8-OHdG) in liver. The results of the study indicated that there was no cytotoxicity (up to 600μg/mL) and oxidative damage (up to 100μg/mL), when exposed to HANPs. It was also evident that, there was no skin sensitization and oxidative damage when HANP were exposed to Guinea pigs.