Improving reliability of live/dead cell counting through automated image mosaicing

Highlights • We present a new two-stage image-based approach to perform cell counting. • Counting is performed on big high-resolution images of the whole hemocytometer's grid. • Counting the cells by also tagging the big image improves counting reliability. • Big images are built using GridMos...

Full description

Saved in:
Bibliographic Details
Published in:Computer methods and programs in biomedicine 2014-12, Vol.117 (3), p.448-463
Main Authors: Piccinini, Filippo, Tesei, Anna, Paganelli, Giulia, Zoli, Wainer, Bevilacqua, Alessandro
Format: Article
Language:English
Subjects:
Algorithms
Applied sciences
Artificial intelligence
Automatic Data Processing
Automation
Biological and medical sciences
Cancer treatments
Cell Count - instrumentation
Cell Count - methods
Cell counting
Cell Line, Tumor
Computer science
control theory
systems
Construction
Counting
Editors
Exact sciences and technology
Hematology
Hemocytometer
Humans
Image processing
Image Processing, Computer-Assisted - instrumentation
Image Processing, Computer-Assisted - methods
Internal Medicine
Investigative techniques, diagnostic techniques (general aspects)
Lung Neoplasms - diagnosis
Lung Neoplasms - pathology
Medical sciences
Microscopy - methods
Mosaics
Oncology
Other
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pattern recognition. Digital image processing. Computational geometry
Repeatability
Reproducibility
Reproducibility of Results
Software
Stomach Neoplasms - diagnosis
Stomach Neoplasms - pathology
Treatment. General aspects
Trypan Blue - chemistry
Tumors
User-Computer Interface
Widefield microscopy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Highlights • We present a new two-stage image-based approach to perform cell counting. • Counting is performed on big high-resolution images of the whole hemocytometer's grid. • Counting the cells by also tagging the big image improves counting reliability. • Big images are built using GridMos , our fully-automated mosaicing software. • Both GridMos source code and a standalone executable version are freely available.
ISSN:0169-2607
1872-7565
DOI:10.1016/j.cmpb.2014.09.004