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Improving reliability of live/dead cell counting through automated image mosaicing
Highlights • We present a new two-stage image-based approach to perform cell counting. • Counting is performed on big high-resolution images of the whole hemocytometer's grid. • Counting the cells by also tagging the big image improves counting reliability. • Big images are built using GridMos...
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| Published in: | Computer methods and programs in biomedicine 2014-12, Vol.117 (3), p.448-463 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c507t-7a7b1041cb3d0accc8dbb98d0f2b26907ed916aebc07a01f71a1eff4126007323 |
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| cites | cdi_FETCH-LOGICAL-c507t-7a7b1041cb3d0accc8dbb98d0f2b26907ed916aebc07a01f71a1eff4126007323 |
| container_end_page | 463 |
| container_issue | 3 |
| container_start_page | 448 |
| container_title | Computer methods and programs in biomedicine |
| container_volume | 117 |
| creator | Piccinini, Filippo Tesei, Anna Paganelli, Giulia Zoli, Wainer Bevilacqua, Alessandro |
| description | Highlights • We present a new two-stage image-based approach to perform cell counting. • Counting is performed on big high-resolution images of the whole hemocytometer's grid. • Counting the cells by also tagging the big image improves counting reliability. • Big images are built using GridMos , our fully-automated mosaicing software. • Both GridMos source code and a standalone executable version are freely available. |
| doi_str_mv | 10.1016/j.cmpb.2014.09.004 |
| format | article |
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Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pattern recognition. Digital image processing. Computational geometry</subject><subject>Repeatability</subject><subject>Reproducibility</subject><subject>Reproducibility of Results</subject><subject>Software</subject><subject>Stomach Neoplasms - diagnosis</subject><subject>Stomach Neoplasms - pathology</subject><subject>Treatment. 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| ispartof | Computer methods and programs in biomedicine, 2014-12, Vol.117 (3), p.448-463 |
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| language | eng |
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| source | Elsevier |
| subjects | Algorithms Applied sciences Artificial intelligence Automatic Data Processing Automation Biological and medical sciences Cancer treatments Cell Count - instrumentation Cell Count - methods Cell counting Cell Line, Tumor Computer science control theory systems Construction Counting Editors Exact sciences and technology Hematology Hemocytometer Humans Image processing Image Processing, Computer-Assisted - instrumentation Image Processing, Computer-Assisted - methods Internal Medicine Investigative techniques, diagnostic techniques (general aspects) Lung Neoplasms - diagnosis Lung Neoplasms - pathology Medical sciences Microscopy - methods Mosaics Oncology Other Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pattern recognition. Digital image processing. Computational geometry Repeatability Reproducibility Reproducibility of Results Software Stomach Neoplasms - diagnosis Stomach Neoplasms - pathology Treatment. General aspects Trypan Blue - chemistry Tumors User-Computer Interface Widefield microscopy |
| title | Improving reliability of live/dead cell counting through automated image mosaicing |
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