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Fluorescent carbonaceous nanospheres as biological probe for noninvasive brain imaging

[Display omitted] •A new kind of fluorescent carbonaceous nanospheres (CDs) was prepared using glutamic acid and glucose as the precursors.•The prepared CDs possessed excellent optical properties and good biocompatibility.•CDs were used as biological probe for cell imaging in vitro.•We report the ap...

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Published in:Journal of colloid and interface science 2014-12, Vol.436 (436), p.227-233
Main Authors: Qian, Jun, Ruan, Shaobo, Cao, Xi, Cun, Xingli, Chen, Jiantao, Shen, Shun, Jiang, Xinguo, He, Qin, Zhu, Jianhua, Gao, Huile
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cited_by cdi_FETCH-LOGICAL-c419t-e6ecd323cde1286c39ac45d5da5615493bcc135d90bd25121075e304caf36ab93
cites cdi_FETCH-LOGICAL-c419t-e6ecd323cde1286c39ac45d5da5615493bcc135d90bd25121075e304caf36ab93
container_end_page 233
container_issue 436
container_start_page 227
container_title Journal of colloid and interface science
container_volume 436
creator Qian, Jun
Ruan, Shaobo
Cao, Xi
Cun, Xingli
Chen, Jiantao
Shen, Shun
Jiang, Xinguo
He, Qin
Zhu, Jianhua
Gao, Huile
description [Display omitted] •A new kind of fluorescent carbonaceous nanospheres (CDs) was prepared using glutamic acid and glucose as the precursors.•The prepared CDs possessed excellent optical properties and good biocompatibility.•CDs were used as biological probe for cell imaging in vitro.•We report the application of CDs in non-invasive brain imaging. Fluorescent carbonaceous nanospheres (CDs) have generated much excitement in bioimaging because of their impressive fluorescent properties and good biocompatibility. In this study, we evaluated the potential application of CDs in noninvasive brain imaging. A new kind of CDs was prepared by a heat treating method using glutamic acid and glucose as the precursors. The hydrated diameter and zeta potential of CDs were 101.1nm (PDI=0.110) and −22.4mV respectively. Palpable emission spectrum could be observed from 400nm to 600nm when excited at corresponding wavelength, suggesting CDs could be used as a noninvasive bio-probe for in vivo imaging. Additionally, several experiments indicated that CDs possess good serum stability and hemocompatibility with low cytotoxicity. In vitro, the CDs could be efficiently taken up by bEnd.3 cells in a concentration- and time-dependent manner. In vivo, CDs could be used for noninvasive brain imaging due to its high accumulation in brain region, which was demonstrated by in vivo imaging and ex vivo tissue imaging. Moreover, the fluorescent distribution in tissue slice showed CDs accumulated in brain with high intensity. In conclusion, CDs were prepared using a simple one-step method with unique optical and good biological properties and could be used for noninvasive brain imaging.
doi_str_mv 10.1016/j.jcis.2014.08.059
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Fluorescent carbonaceous nanospheres (CDs) have generated much excitement in bioimaging because of their impressive fluorescent properties and good biocompatibility. In this study, we evaluated the potential application of CDs in noninvasive brain imaging. A new kind of CDs was prepared by a heat treating method using glutamic acid and glucose as the precursors. The hydrated diameter and zeta potential of CDs were 101.1nm (PDI=0.110) and −22.4mV respectively. Palpable emission spectrum could be observed from 400nm to 600nm when excited at corresponding wavelength, suggesting CDs could be used as a noninvasive bio-probe for in vivo imaging. Additionally, several experiments indicated that CDs possess good serum stability and hemocompatibility with low cytotoxicity. In vitro, the CDs could be efficiently taken up by bEnd.3 cells in a concentration- and time-dependent manner. In vivo, CDs could be used for noninvasive brain imaging due to its high accumulation in brain region, which was demonstrated by in vivo imaging and ex vivo tissue imaging. Moreover, the fluorescent distribution in tissue slice showed CDs accumulated in brain with high intensity. In conclusion, CDs were prepared using a simple one-step method with unique optical and good biological properties and could be used for noninvasive brain imaging.</description><identifier>ISSN: 0021-9797</identifier><identifier>EISSN: 1095-7103</identifier><identifier>DOI: 10.1016/j.jcis.2014.08.059</identifier><identifier>PMID: 25278360</identifier><identifier>CODEN: JCISA5</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Animals ; Biocompatibility ; Biocompatible Materials ; Biological ; Biological probe ; Biomedical materials ; Brain ; Brain imaging ; Carbon - chemistry ; Chemistry ; Colloidal state and disperse state ; Diagnostic Imaging ; Exact sciences and technology ; Fluorescent carbonaceous nanospheres ; Fluorescent Dyes - chemistry ; General and physical chemistry ; Imaging ; In vivo testing ; In vivo tests ; Male ; Mice ; Nanospheres - chemistry ; Physical and chemical studies. Granulometry. 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Fluorescent carbonaceous nanospheres (CDs) have generated much excitement in bioimaging because of their impressive fluorescent properties and good biocompatibility. In this study, we evaluated the potential application of CDs in noninvasive brain imaging. A new kind of CDs was prepared by a heat treating method using glutamic acid and glucose as the precursors. The hydrated diameter and zeta potential of CDs were 101.1nm (PDI=0.110) and −22.4mV respectively. Palpable emission spectrum could be observed from 400nm to 600nm when excited at corresponding wavelength, suggesting CDs could be used as a noninvasive bio-probe for in vivo imaging. Additionally, several experiments indicated that CDs possess good serum stability and hemocompatibility with low cytotoxicity. In vitro, the CDs could be efficiently taken up by bEnd.3 cells in a concentration- and time-dependent manner. In vivo, CDs could be used for noninvasive brain imaging due to its high accumulation in brain region, which was demonstrated by in vivo imaging and ex vivo tissue imaging. Moreover, the fluorescent distribution in tissue slice showed CDs accumulated in brain with high intensity. In conclusion, CDs were prepared using a simple one-step method with unique optical and good biological properties and could be used for noninvasive brain imaging.</description><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials</subject><subject>Biological</subject><subject>Biological probe</subject><subject>Biomedical materials</subject><subject>Brain</subject><subject>Brain imaging</subject><subject>Carbon - chemistry</subject><subject>Chemistry</subject><subject>Colloidal state and disperse state</subject><subject>Diagnostic Imaging</subject><subject>Exact sciences and technology</subject><subject>Fluorescent carbonaceous nanospheres</subject><subject>Fluorescent Dyes - chemistry</subject><subject>General and physical chemistry</subject><subject>Imaging</subject><subject>In vivo testing</subject><subject>In vivo tests</subject><subject>Male</subject><subject>Mice</subject><subject>Nanospheres - chemistry</subject><subject>Physical and chemical studies. 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subjects Animals
Biocompatibility
Biocompatible Materials
Biological
Biological probe
Biomedical materials
Brain
Brain imaging
Carbon - chemistry
Chemistry
Colloidal state and disperse state
Diagnostic Imaging
Exact sciences and technology
Fluorescent carbonaceous nanospheres
Fluorescent Dyes - chemistry
General and physical chemistry
Imaging
In vivo testing
In vivo tests
Male
Mice
Nanospheres - chemistry
Physical and chemical studies. Granulometry. Electrokinetic phenomena
Surgical implants
title Fluorescent carbonaceous nanospheres as biological probe for noninvasive brain imaging
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