Use of novel immobilized beta-galactosidase reactor to hydrolyze the lactose constituent of skim milk. II. Multiresponse nonlinear regression analysis of kinetic data

A novel chemical reactor, consisting of beta -galactosidase from Aspergillus oryzae immobilized on a ribbed membrane made from polyvinylchloride and silica, was used to hydrolyze the lactose constituent of skim milk. Multiresponse nonlinear regression methods were employed to determine the kinetic p...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1991-01, Vol.28 (1), p.741-756
Main Authors: Bakken, A.P. (University of Wisconsin, Madison, WI), Hill, C.G. Jr, Amundson, C.H
Format: Article
Language:English
Subjects:
ANALISIS ESTADISTICO
ANALYSE STATISTIQUE
Aspergillus oryzae
BETA GALACTOSIDASA
BETA GALACTOSIDASE
Biological and medical sciences
BIOREACTEUR
BIOREACTORS
BIORREACTORES
Biotechnology
Fundamental and applied biological sciences. Psychology
IMMOBILISATION
IMMOBILIZATION
INMOVILIZACION
LACTOSA
LACTOSE
LAIT ECREME
LECHE DESNATADA
Methods. Procedures. Technologies
MULTIRESPONSE REGRESSION
SKIM MILK
STATISTICAL ANALYSIS
Various methods and equipments
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Summary:A novel chemical reactor, consisting of beta -galactosidase from Aspergillus oryzae immobilized on a ribbed membrane made from polyvinylchloride and silica, was used to hydrolyze the lactose constituent of skim milk. Multiresponse nonlinear regression methods were employed to determine the kinetic parameters of rate expressions based on a proposed enzymatic mechanism that includes the formation of oligosaccharides. HPLC methods were employed to monitor the concentrations of all species present in the effluent stream. For the experimental conditions used in this research, a rate expression that includes the inhibition effect of alpha -galactose is sufficient to model the reaction network.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02922646