Immunocytochemical localization of photosystem I and the fucoxanthin-chlorophyll a/c light-harvesting complex in the diatom Phaeodactylum tricornutum

Antiserum against two polypeptides of the major fucoxanthin-chlorophyll a/c light-harvesting complex of the diatom Phaeodactylum tricornutum and heterologous antiserum against purified photosystem I particles of maize were used to localize these two complexes on the thylakoid membranes of P. tricorn...

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Bibliographic Details
Published in:Protoplasma 1992-01, Vol.166 (3-4), p.208-217
Main Authors: PYSZNIAK, A. M, GIBBS, S. P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell structures and functions
Chloroplast, photosynthetic membrane and photosynthesis
Fundamental and applied biological sciences. Psychology
Metabolism
Molecular and cellular biology
Phaeodactylum tricornutum
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
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Summary:Antiserum against two polypeptides of the major fucoxanthin-chlorophyll a/c light-harvesting complex of the diatom Phaeodactylum tricornutum and heterologous antiserum against purified photosystem I particles of maize were used to localize these two complexes on the thylakoid membranes of P. tricornutum . Immunoelectron microscopy demonstrated that the fucoxanthin-chlorophyll a/c light-harvesting complex, which is believed to be associated with photosystem II, was equally distributed on the appressed and non-appressed thylakoid membranes.
ISSN:0033-183X
1615-6102
DOI:10.1007/bf01322783