Development and validation of an ultra-performance liquid chromatography method for simultaneous analysis of 20 antihistaminics in dietary supplements

The purpose of this study was to develop and validate an ultra‐performance liquid chromatography method for simultaneous analysis of 20 antihistamines (illegal additives) in dietary supplements. The limits of detection and quantitation of the method ranged from 1.5 to 2.5 µg/mL and from 20.0 to 50.0...

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Bibliographic Details
Published in:Biomedical chromatography 2015-03, Vol.29 (3), p.465-474
Main Authors: Kim, Jung Yeon, Do, Jung-Ah, Choi, Ji Yeon, Cho, Sooyeul, Kim, Woo-Seong, Yoon, Chang-Yong
Format: Article
Language:English
Subjects:
antihistamine
Chromatography, Liquid - methods
dietary supplement
Dietary Supplements - analysis
Drug Stability
Histamine Antagonists - analysis
Sensitivity and Specificity
UPLC
validated
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Summary:The purpose of this study was to develop and validate an ultra‐performance liquid chromatography method for simultaneous analysis of 20 antihistamines (illegal additives) in dietary supplements. The limits of detection and quantitation of the method ranged from 1.5 to 2.5 µg/mL and from 20.0 to 50.0 µg/mL, respectively. The determination coefficient was >0.999, precisions were 0.2–5.1% (intra‐day) and 0.1–8.8% (inter‐day), and accuracies were 84.5–111.2% (intra‐day) and 91.9–112.0% (inter‐day). The mean recoveries of 20 targeted compounds from dietary supplements ranged from 75.4 to 119.3%. The relative standard deviations were
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3298