In ovo carbohydrate supplementation modulates growth and immunity‐related genes in broiler chickens

• Published in: Journal of animal physiology and animal nutrition 2015-02, Vol.99 (1), p.163-173
• Main Authors: Bhanja, S. K, Goel, A, Pandey, N, Mehra, M, Majumdar, S, Mandal, A. B
• Format: Article
• Language: English
• Subjects: amnion; Animals; broiler chickens; carbohydrates; Carbohydrates - administration & dosage; Carbohydrates - pharmacology; cell-mediated immunity; Chick Embryo; Chickens - growth & development; Chickens - immunology; chicks; concanavalin A; erythrocytes; fructose; gene expression; Gene Expression Regulation - drug effects; Gene Expression Regulation - immunology; genes; glucose; growth and immunity; in ovo injection; insulin-like growth factor I; insulin-like growth factor II; interferon-gamma; interleukin-10; interleukin-12; interleukin-2; interleukin-6; jejunum; monocytes; mucins; Ovum; ribose; sheep; somatotropin; tissues; tumor necrosis factor-alpha; yolk sac
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/jpn.12193

A study was undertaken to investigate the role of in ovo administrated carbohydrates on the expression pattern of growth and immune‐related genes. In ovo injections (n = 400) were carried out on the 14th day of incubation into the yolk sac/amnion of the broiler chicken embryos. Expression of growth‐related genes: chicken growth hormone (cGH), insulin‐like growth factor‐I & II (IGF‐I & II) and mucin were studied in hepatic and jejunum tissues of late‐term embryo and early post‐hatch chicks. Expression of candidate immune genes: Interleukin‐2, 6, 10 and 12 (IL‐2, IL‐6, IL‐10 and IL‐12), Tumour necrosis factor‐alpha (TNF‐α) and Interferon gamma (IFN‐γ) were studied in peripheral blood monocyte cells of in ovo‐injected and control birds following antigenic stimulation with sheep RBC (SRBC) or mitogen concanavalin A (Con‐A). Glucose injection significantly increased the expression of IGF‐II gene during embryonic period and both cGH and IGF‐II in early post‐hatch period, while ribose‐injected chicks had higher expression of IGF‐II gene during embryonic stage. Enhanced mucin gene expression was also observed in fructose‐injected chicks during embryonic age. Glucose‐injected chicks had higher expression of IL‐6 or IL‐10, while those injected with fructose or ribose had higher expression of IL‐2, IL‐12 and IFN gamma. It is concluded that in ovo supplementation of carbohydrates might help in improving the growth of late‐term embryos and chicks. In ovo glucose could modulate humoral‐related immunity, while fructose or ribose might help in improving the cellular immunity in broiler chickens.