"YFlag" - A Single-base Extension Primer Based Method for Gender Determination

Assigning the gender of a DNA contributor in forensic analysis is typically achieved using the amelogenin test. Occasionally, this test produces false‐positive results due to deletions occurring on the Y chromosome. Here, a four‐marker “YFlag” method is presented to infer gender using single‐base ex...

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Bibliographic Details
Published in:Journal of forensic sciences 2015-01, Vol.60 (1), p.142-146
Main Authors: Allwood, Julia S., Harbison, Sally Ann
Format: Article
Language:English
Subjects:
amelogenin
Cell Cycle Proteins - genetics
Chromosomes, Human, Y - genetics
Deoxyribonucleic acid
DNA
DNA analysis
DNA Fingerprinting
DNA Primers
Female
forensic science
Forensic sciences
Gender
Genes, sry - genetics
Genetic Markers
Humans
Male
Multiplex Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Proteins - genetics
Reproducibility of Results
sex determination
Sex Determination Analysis - methods
single-base extension
Y chromosome
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Summary:Assigning the gender of a DNA contributor in forensic analysis is typically achieved using the amelogenin test. Occasionally, this test produces false‐positive results due to deletions occurring on the Y chromosome. Here, a four‐marker “YFlag” method is presented to infer gender using single‐base extension primers to flag the presence (or absence) of Y‐chromosome DNA within a sample to supplement forensic STR profiling. This method offers built‐in redundancy, with a single marker being sufficient to detect the presence of male DNA. In a study using 30 male and 30 female individuals, detection of male DNA was achieved with c. 0.03 ng of male DNA. All four markers were present in male/female mixture samples despite the presence of excessive female DNA. In summary, the YFlag system offers a method that is reproducible, specific, and sensitive, making it suitable for forensic use to detect male DNA.
ISSN:0022-1198
1556-4029
DOI:10.1111/1556-4029.12553