Loading…
The Chinese herb polyphyllin D sensitizes ovarian cancer cells to cisplatin-induced growth arrest
Purpose We evaluated the effects of polyphyllin D (PD), a natural compound with anti-neoplastic activity and a major component of the Chinese herb Paris polyphylla , on ovarian cancer (OVCA) cell line proliferation and platinum sensitivity. Methods A panel of 20 OVCA cell lines was subjected to PD t...
Saved in:
Published in: | Journal of cancer research and clinical oncology 2015-02, Vol.141 (2), p.237-242 |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Purpose
We evaluated the effects of polyphyllin D (PD), a natural compound with anti-neoplastic activity and a major component of the Chinese herb
Paris polyphylla
, on ovarian cancer (OVCA) cell line proliferation and platinum sensitivity.
Methods
A panel of 20 OVCA cell lines was subjected to PD treatment, MTS proliferation assays, and determination of IC
50
. Pre-treatment, baseline genome-wide Affymetrix expression analysis was performed on each cell line, and Pearson’s correlation was performed to identify genes associated with OVCA PD sensitivity. Twelve cell lines were treated with PD with and without cisplatin, and the effects of PD on cisplatin IC
50
were quantified. Genes associated with OVCA PD sensitivity were evaluated for associations with survival in a publically available clinico-genomic dataset of 218 patients with OVCA.
Results
Our results showed that PD exhibited anti-proliferative effects against all OVCA cell lines tested, with IC
50
values ranging from 0.2 to 1.4 μm. Furthermore, in all cell lines, PD treatment significantly decreased cisplatin IC
50
(mean IC
50
reduction of 2.1 µm;
P
|
---|---|
ISSN: | 0171-5216 1432-1335 |
DOI: | 10.1007/s00432-014-1797-x |