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The Chinese herb polyphyllin D sensitizes ovarian cancer cells to cisplatin-induced growth arrest

Purpose We evaluated the effects of polyphyllin D (PD), a natural compound with anti-neoplastic activity and a major component of the Chinese herb Paris polyphylla , on ovarian cancer (OVCA) cell line proliferation and platinum sensitivity. Methods A panel of 20 OVCA cell lines was subjected to PD t...

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Published in:Journal of cancer research and clinical oncology 2015-02, Vol.141 (2), p.237-242
Main Authors: Sawah, Entidhar Al, Marchion, Douglas C., Xiong, Yin, Ramirez, Ingrid J., Abbasi, Forough, Boac, Bernadette M., Bush, Stephen H., Zgheib, Nadim Bou, McClung, E. Clair, Khulpateea, Beman R., Berry, Amy, Hakam, Ardeshir, Wenham, Robert M., Lancaster, Johnathan M., Judson, Patricia L.
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Language:English
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Summary:Purpose We evaluated the effects of polyphyllin D (PD), a natural compound with anti-neoplastic activity and a major component of the Chinese herb Paris polyphylla , on ovarian cancer (OVCA) cell line proliferation and platinum sensitivity. Methods A panel of 20 OVCA cell lines was subjected to PD treatment, MTS proliferation assays, and determination of IC 50 . Pre-treatment, baseline genome-wide Affymetrix expression analysis was performed on each cell line, and Pearson’s correlation was performed to identify genes associated with OVCA PD sensitivity. Twelve cell lines were treated with PD with and without cisplatin, and the effects of PD on cisplatin IC 50 were quantified. Genes associated with OVCA PD sensitivity were evaluated for associations with survival in a publically available clinico-genomic dataset of 218 patients with OVCA. Results Our results showed that PD exhibited anti-proliferative effects against all OVCA cell lines tested, with IC 50 values ranging from 0.2 to 1.4 μm. Furthermore, in all cell lines, PD treatment significantly decreased cisplatin IC 50 (mean IC 50 reduction of 2.1 µm; P  
ISSN:0171-5216
1432-1335
DOI:10.1007/s00432-014-1797-x