Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil

[Display omitted] •An immunosensor based on MWCNTs/ILs/Ab is presented to test aflatoxin B1.•MWCNTs/ILs possesses 3D structure, excellent conductivity and biocompatibility.•Recombinant antibodies are sensitive to low molecular weight haptens.•Electrochemical impedance spectroscopy (EIS) is sensitive...

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Bibliographic Details
Published in:Food chemistry 2015-06, Vol.176, p.22-26
Main Authors: Yu, Lili, Zhang, Yang, Hu, Chenyi, Wu, Hui, Yang, Yayun, Huang, Chusen, Jia, Nengqin
Format: Article
Language:English
Subjects:
Aflatoxin B1 (AFB1)
Aflatoxin B1 - analysis
Biosensing Techniques - methods
Carbon nanotube
Dielectric Spectroscopy - methods
Electrochemical impedance spectroscopy (EIS)
Immunosensor
Limit of Detection
Olive Oil
Plant Oils - chemistry
Room temperature ionic liquids (RTILs)
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Summary:[Display omitted] •An immunosensor based on MWCNTs/ILs/Ab is presented to test aflatoxin B1.•MWCNTs/ILs possesses 3D structure, excellent conductivity and biocompatibility.•Recombinant antibodies are sensitive to low molecular weight haptens.•Electrochemical impedance spectroscopy (EIS) is sensitive, convenient and economic.•AFB1 can be analyzed quantitatively in real samples (e.g. olive oil). Aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus are commonly found in olive and its derivatives. Aflatoxin B1 (AFB1) is a predominant toxin detected abundantly and has been implicated in the etiology of human hepatocellular carcinoma. This study proposes a sensitive and convenient electrochemical impedance spectroscopy (EIS) method for determining AFB1 by MWCNTs/RTIL composite films-based immunosensor. The calibration curve for AFB1 was linear in the range of 0.1–10ngmL−1 with the limit of detection (LOD) 0.03ngmL−1. The presence of MWCNTs warrant fast electron transfer, and the ionic liquid provides a benign microenvironment for antibody. The experimental parameters, such as pH and incubating time, have been investigated and optimized. Furthermore, the detection of AFB1 is presented to test this method after extracted from olive oils. It can be anticipated that this method would be used for the detection of AFB1 in various agriculture products and vegetable oils.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2014.12.030