Adipose-derived stem cells incorporated into platelet-rich plasma improved bone regeneration and maturation in vivo

Background/Aim Some cases of tooth loss related to dental trauma require bone‐grafting procedures to improve the aesthetics before prosthetic rehabilitation or to enable the installation of dental implants. Bone regeneration is often a challenge and could be largely improved by mesenchymal stem cell...

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Bibliographic Details
Published in:Dental traumatology 2015-02, Vol.31 (1), p.42-48
Main Authors: Cruz, Ariadne Cristiane Cabral, Caon, Thiago, Menin, Álvaro, Granato, Rodrigo, Boabaid, Fernanda, Simões, Cláudia Maria Oliveira
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Animals
bone regeneration
Bone Regeneration - physiology
Bone Transplantation - methods
Cell Differentiation
Cell Survival
Dentistry
Dogs
Humans
Immunophenotyping
mesenchymal stem cells
Mesenchymal Stromal Cells - physiology
osteogenesis
Platelet-Rich Plasma
Tibia - surgery
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Summary:Background/Aim Some cases of tooth loss related to dental trauma require bone‐grafting procedures to improve the aesthetics before prosthetic rehabilitation or to enable the installation of dental implants. Bone regeneration is often a challenge and could be largely improved by mesenchymal stem cells therapy. However, the appropriate scaffold for these cells still a problem. This study evaluated the in vivo effect of human adipose‐derived stem cells incorporated into autogenous platelet‐rich plasma in bone regeneration and maturation. Material and methods Adipose‐derived stem cells were isolated from lipoaspirate tissues and used at passage 4. Immunophenotyping and multilineage differentiation of cells were performed and mesenchymal stem cells characteristics confirmed. Bicortical bone defects (10 mm diameter) were created in the tibia of six beagle dogs to evaluate the effect of adipose‐derived stem cells incorporated into platelet‐rich plasma scaffolds, platelet‐rich plasma alone, autogenous bone grafts, and clot. Samples were removed 6 weeks postsurgeries and analyzed by quantification of primary and secondary bone formation and granulation tissue. Results Adipose‐derived stem cells incorporated into platelet‐rich plasma scaffolds promoted the highest bone formation (primary + secondary bone) (P 
ISSN:1600-4469
1600-9657
DOI:10.1111/edt.12134