Regulatory functions of evolutionarily conserved AN1/A20-like Zinc finger family proteins in Arabidopsis stress responses under high temperature

•The Arabidopsis A20/AN1 domain protein AtSAP5 is localized to the nucleus.•AtSAP5 modulates heat stress-related HSPs together with MBF1c.•Two independent sap5 mutants compromised two-step heat stress tolerance. AN1/A20-like Zinc finger family proteins are evolutionarily conserved regulatory compone...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2015-02, Vol.457 (2), p.213-220
Main Authors: Kim, Geun-Don, Cho, Young-Hee, Yoo, Sang-Dong
Format: Article
Language:English
Subjects:
A20/AN1 zinc finger protein
Adaptation, Physiological - genetics
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis Proteins - chemistry
Arabidopsis Proteins - metabolism
Cell Nucleus - metabolism
Conserved Sequence
Evolution, Molecular
Gene Expression Regulation, Plant
Genes, Plant
Heat stress tolerance
Hot Temperature
MBF1c
Protein Binding
Protein Transport
Stress, Physiological - genetics
Trans-Activators - metabolism
Transcription, Genetic
Ubiquitin-Protein Ligases - chemistry
Ubiquitin-Protein Ligases - metabolism
Zinc Fingers
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Summary:•The Arabidopsis A20/AN1 domain protein AtSAP5 is localized to the nucleus.•AtSAP5 modulates heat stress-related HSPs together with MBF1c.•Two independent sap5 mutants compromised two-step heat stress tolerance. AN1/A20-like Zinc finger family proteins are evolutionarily conserved regulatory components in eukaryotic signaling circuits. In Arabidopsis thaliana, the AN1/A20 Zinc finger family is encoded as 14 members in the genome and collectively referred to as stress-associated proteins (SAPs). Here we described AtSAP5 localized to the nucleus, and played a role in heat-responsive gene regulation together with MBF1c. Seedling survival assay of sap5 and mbf1c demonstrated consistent effects of AtSAP5 and MBF1C in response to two-step heat treatment, supporting their function in heat stress tolerance. Our findings yield an insight in A20/AN1-like Zinc finger protein AtSAP5 functions in plant adaptability under high temperature.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2014.12.090