A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide

Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investi...

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Bibliographic Details
Published in:Organic & biomolecular chemistry 2015-02, Vol.13 (6), p.1763-1767
Main Authors: Bag, S, Tseng, J-C, Rochford, J
Format: Article
Language:English
Subjects:
Animals
Boron Compounds - chemistry
Fluorescence Resonance Energy Transfer
Luminescent Measurements
Luminol - chemistry
Mice
Mice, Nude
Molecular Structure
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Spleen - chemistry
Spleen - cytology
Superoxides - chemistry
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Summary:Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET investigated for the BODIPY-luminol dyad was found to be 64% resulting in a dual emissive response. Successful in cellulo biochemiluminescence via CRET was achieved in PMA activated splenocytes.
ISSN:1477-0520
1477-0539
DOI:10.1039/c4ob02413c