Quantitative Measurement of Toll-like Receptor 4 Agonists Adsorbed to Alhydrogel® by Fourier Transform Infrared-Attenuated Total Reflectance Spectroscopy

Aluminum salts have a long history as safe and effective vaccine adjuvants. In addition, aluminum salts have high adsorptive capacities for vaccine antigens and adjuvant molecules, for example, Toll-like receptor 4 (TLR4) agonists. However, the physicochemical properties of aluminum salts make direc...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 2015-02, Vol.104 (2), p.768-774
Main Authors: Dowling, Quinton M., Schwartz, Alicia M., Vedvick, Thomas S., Fox, Christopher B., Kramer, Ryan M.
Format: Article
Language:English
Subjects:
Absorption Spectroscopy
Adsorption
Aluminum Hydroxide - analysis
Aluminum Hydroxide - metabolism
aluminum hydroxide adjuvant
FTIR
glucopyranosyl lipid adjuvant (GLA)
Glucosides - analysis
Glucosides - metabolism
hydrogels
Lipid A - analysis
Lipid A - metabolism
Spectroscopy, Fourier Transform Infrared - methods
Toll-Like Receptor 4 - agonists
vaccine adjuvants
vaccines
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Summary:Aluminum salts have a long history as safe and effective vaccine adjuvants. In addition, aluminum salts have high adsorptive capacities for vaccine antigens and adjuvant molecules, for example, Toll-like receptor 4 (TLR4) agonists. However, the physicochemical properties of aluminum salts make direct quantitation of adsorbed molecules challenging. Typical methods for quantifying adsorbed molecules require advanced instrumentation, extreme sample processing, often destroy the sample, or rely on an indirect measurement. A simple, direct, and quantitative method for analysis of adsorbed adjuvant molecules is needed. This report presents a method utilizing Fourier transform infrared spectroscopy with a ZnSe-attenuated total reflectance attachment to directly measure low levels (
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.24180