A Simultaneous Determination of Principal Compounds in Tokishakuyakusan by High-Performance Liquid Chromatography with Diode Array Detector

We developed a simultaneous analysis method using high-performance liquid chromatography coupled with diode-array detector (HPLC-DAD) for six principal compounds (atractylenolide III, alisol A, alisol B, paeoniflorin, ferulic acid and (Z)-ligustilide) in a traditional Japanese (Kampo) medicine, toki...

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Bibliographic Details
Published in:Journal of chromatographic science 2015-02, Vol.53 (2), p.320-324
Main Authors: Sumino, Megumi, Saito, Yuko, Ikegami, Fumio, Namiki, Takao
Format: Article
Language:English
Subjects:
4-Butyrolactone - analogs & derivatives
4-Butyrolactone - analysis
4-Butyrolactone - chemistry
Cholestenones - analysis
Cholestenones - chemistry
Chromatography, High Pressure Liquid - methods
Coumaric Acids - analysis
Coumaric Acids - chemistry
Drugs, Chinese Herbal - analysis
Drugs, Chinese Herbal - chemistry
Glucosides - analysis
Glucosides - chemistry
Lactones - analysis
Lactones - chemistry
Limit of Detection
Linear Models
Monoterpenes - analysis
Monoterpenes - chemistry
Reproducibility of Results
Sesquiterpenes - analysis
Sesquiterpenes - chemistry
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Summary:We developed a simultaneous analysis method using high-performance liquid chromatography coupled with diode-array detector (HPLC-DAD) for six principal compounds (atractylenolide III, alisol A, alisol B, paeoniflorin, ferulic acid and (Z)-ligustilide) in a traditional Japanese (Kampo) medicine, tokishakuyakusan (TSS). The HPLC separation was conducted on a reversed-phase TSK-gel ODS-80TS column (4.6 i.d. × 250 mm, 5 µm) at 40°C with a 0.1% phosphoric acid–acetonitrile gradient system. The DAD detection wavelength was set at 205, 232 and 330 nm. Calibration curves for the compounds showed linear regressions with correlation coefficients of >0.999. The intra- and inter-day precision (i.e., the relative standard deviation) were in the range of 0.50–1.55 and 0.70–1.80%, respectively. The average recovery yields of the compounds ranged from 98.3 to 103%. The present results will contribute to shorter analysis times with less organic solvent compared with the individual analysis of each compound for the evaluation of TSS. The application of the established method to TSS will also provide helpful information for the further pharmacological and clinical studies.
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmu062