Experimental study of the relationship between Plasmodium gametocyte density and infection success in mosquitoes; implications for the evaluation of malaria transmission-reducing interventions

• Published in: Experimental parasitology 2015-02, Vol.149, p.74-83
• Main Authors: Da, Dari F., Churcher, Thomas S., Yerbanga, Rakiswendé S., Yaméogo, Bienvenue, Sangaré, Ibrahim, Ouedraogo, Jean Bosco, Sinden, Robert E., Blagborough, Andrew M., Cohuet, Anna
• Format: Article
• Language: English
• Subjects: Animals; Anopheles; Anopheles - parasitology; Carrier State - parasitology; Child; Child, Preschool; Dilution; Female; Gametocyte density; Humans; Infection success; Insect Vectors - parasitology; Malaria - parasitology; Malaria - transmission; Malaria, Falciparum - parasitology; Malaria, Falciparum - transmission; Mice; Plasmodium; Plasmodium berghei - growth & development; Plasmodium falciparum - growth & development; Transmission blocking interventions
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2014.12.010

•Infection outcome in mosquito increases with Plasmodium falciparum gametocyte density.•At high Plasmodium berghei gametocyte densities, infection outcome in mosquito decreases.•Reduction of infection intensity by mAb 13.1 was constant over oocyst intensities.•Reduction of infection prevalence by mAb 13.1 decreases at high infection loads.•Evaluation of TRIs should report reduction of infection intensity and prevalence. The evaluation of transmission reducing interventions (TRI) to control malaria widely uses membrane feeding assays. In such assays, the intensity of Plasmodium infection in the vector might affect the measured efficacy of the candidates to block transmission. Gametocyte density in the host blood is a determinant of the infection success in the mosquito, however, uncertain estimates of parasite densities and intrinsic characteristics of the infected blood can induce variability. To reduce this variation, a feasible method is to dilute infectious blood samples. We describe the effect of diluting samples of Plasmodium-containing blood samples to allow accurate relative measures of gametocyte densities and their impact on mosquito infectivity and TRI efficacy. Natural Plasmodium falciparum samples were diluted to generate a wide range of parasite densities, and fed to Anopheles coluzzii mosquitoes. This was compared with parallel dilutions conducted on Plasmodium berghei infections. We examined how blood dilution influences the observed blocking activity of anti-Pbs28 monoclonal antibody using the P. berghei/Anopheles stephensi system. In the natural species combination P. falciparum/An. coluzzii, blood dilution using heat-inactivated, infected blood as diluents, revealed positive near linear relationships, between gametocyte densities and oocyst loads in the range tested. A similar relationship was observed in the P. berghei/An. stephensi system when using a similar dilution method. In contrast, diluting infected mice blood with fresh uninfected blood dramatically increases the infectiousness. This suggests that highly infected mice blood contains inhibitory factors or reduced blood moieties, which impede infection and may in turn, lead to misinterpretation when comparing individual TRI evaluation assays. In the lab system, the transmission blocking activity of an antibody specific for Pbs28 was confirmed to be density-dependent. This highlights the need to carefully interpret evaluations of TRI candidates, regarding gametocyte densities in th