Neuropharmacological effects of carvacryl acetate on δ-aminolevulinic dehydratase, Na+, K+-ATPase activities and amino acids levels in mice hippocampus after seizures

• Published in: Chemico-biological interactions 2015-01, Vol.226, p.49-57
• Main Authors: Pires, Lúcio Fernandes, Costa, Luciana Muratori, de Almeida, Antonia Amanda Cardoso, Silva, Oskar Almeida, Cerqueira, Gilberto Santos, de Sousa, Damião Pergentino, Pires, Rosana Martins Carneiro, Satyal, Prabodh, de Freitas, Rivelilson Mendes
• Format: Article
• Language: English
• Subjects: Acetate; Amino Acids - metabolism; Animals; Anticonvulsant; Carvacryl; Epilepsy; Hippocampus - drug effects; Hippocampus - enzymology; Hippocampus - metabolism; Male; Mice; Monoterpenes - pharmacology; Mouse; Porphobilinogen Synthase - metabolism; Rotarod Performance Test; Seizures - chemically induced; Seizures - enzymology; Seizures - metabolism; Sodium-Potassium-Exchanging ATPase - metabolism
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2014.12.001

[Display omitted] •Anticonvulsant effects of carvacryl acetate (CA) were verified in experimental models.•CA reduced seizures, deaths and increased the latency for first seizure.•CA improved Na+, K+-ATPase and δ-ALA-D activities in mice hippocampus.•CA did not alter locomotor activity and motor coordination of mice. Epileptic syndromes are highly prevalent neurological conditions and can often be disabling. In order to find an alternative for treatment, this study evaluated anticonvulsant effects of carvacryl acetate (CA), a derivative of monoterpene carvacrol, after seizures induced by pilocarpine (P400), picrotoxin (PIC) or pentylenetetrazol (PTZ). We also analyzed the CA effects on Na+, K+-ATPase and δ-aminolevulinic acid dehydratase (δ-ALA-D) activities in hippocampus mice after seizures induced by P400, PIC or PTZ. In addition, glutamate, δ-aminobutyric acid (GABA), glutamine and aspartate levels in mice hippocampus treated with CA after seizures induced by P400, PIC or PTZ were also measured. CA produced anticonvulsant effects against seizures induced by P400, PIC or PTZ, and its effects were reversed by flumazenil, suggesting that action mechanism can be mediated by GABAergic system. CA increased GABA levels, but did not alter glutamate and aspartate concentrations in mice hippocampus after seizures induced by P400, PIC or PTZ when compared with seizures induced by P400, PIC or PTZ (p