Mucosal trauma induced apoptosis in guinea pig middle ear: Comparision of hemostatic agents

• Published in: International journal of pediatric otorhinolaryngology 2014-12, Vol.78 (12), p.2222-2228
• Main Authors: Eren, Erdem, Başoğlu, Mehmet Sinan, Kulduk, Erkan, Şimsek, Fatma, İnan, Sevinç
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; bcl-X Protein - metabolism; Biocompatible Materials - pharmacology; Caspase 3 - metabolism; Ear, Middle - metabolism; Ear, Middle - pathology; Epithelium - pathology; Gelatin Sponge, Absorbable - pharmacology; Guinea Pigs; Hemostatic; Hemostatics - pharmacology; Immunohistochemical; Inflammation; Inflammation - pathology; Microscopy; Models, Animal; Mucous Membrane - injuries; Mucous Membrane - pathology; Otolaryngology; Pediatrics; Plant Extracts - pharmacology; Polysaccharides - pharmacology; Wound Healing - drug effects
• ISSN: 0165-5876; 1872-8464
• DOI: 10.1016/j.ijporl.2014.10.017

Abstract Objective The aim of this study is to compare the effects of the absorbable gelatin sponge (AGS), microporous polysaccharide hemospheres (MPH), and Ankaferd on wound healing after middle ear trauma and to evaluate their ototoxicity in an experimental guinea pig model. Methods Middle ear mucosal trauma was created in 21 healthy adult guinea pigs. MPH, Ankaferd, and AGS were applied into the right tympanic bulla of the guinea pigs (7 ears for each treatment modality). The left ears of the seven animals were used as the sham group. At the fourth postoperative week (28–30 days), the guinea pigs were decapitated. Apoptosis was investigated, and the expression of Bcl-xl, Apaf, p53, cytochrome 3, and caspase 3 were evaluated. Results The Ankaferd and AGS groups demonstrated significantly lower epithelial thickness, inflammation, and capillary dilatation than did the control group ( p < 0.001,