The dtd gene from Bacillus amyloliquefaciens encodes a putative d-tyrosyl-tRNATyr deacylase and is a selectable marker for Bacillus subtilis

Genetically engineered microbes are of high practical importance due to their cost-effective production of valuable metabolites and enzymes, and the search for new selectable markers for genetic manipulation is of particular interest. Here, we revealed that the soil bacterium Bacillus amyloliquefaci...

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Bibliographic Details
Published in:Microbiological research 2015-02, Vol.171, p.90-96
Main Authors: Geraskina, Natalia V., Butov, Ivan A., Yomantas, Yurgis A.V., Stoynova, Nataliya V.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acids - pharmacology
Aminoacyltransferases - chemistry
Aminoacyltransferases - genetics
Aminoacyltransferases - metabolism
Bacillus - drug effects
Bacillus - genetics
Bacillus - metabolism
Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - metabolism
d-tyrosyl-tRNATyr deacylase
Gene Order
Genetic Markers
Molecular Sequence Data
Mutation
Non-antibiotic resistance selection
Open Reading Frames
Plasmids - genetics
Sequence Alignment
yrvI (dtd)
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Summary:Genetically engineered microbes are of high practical importance due to their cost-effective production of valuable metabolites and enzymes, and the search for new selectable markers for genetic manipulation is of particular interest. Here, we revealed that the soil bacterium Bacillus amyloliquefaciens A50 is tolerant to the non-canonical amino acid d-tyrosine (d-Tyr), in contrast to the closely related Bacillus strain B. subtilis 168, which is a widely used “domesticated” laboratory strain. The gene responsible for resistance to d-Tyr was identified. The resistance was associated with the activity of a potential d-tyrosyl-tRNATyr deacylase. Orthologs of this enzyme are capable of hydrolyzing the ester bond and recycling misacetylated d-aminoacyl-tRNA molecules into free tRNAs and d-amino acids. This gene, yrvI (dtd), is applicable as a convenient, small selectable marker for non-antibiotic resistance selection in experiments aimed at genome editing of d-Tyr-sensitive microorganisms.
ISSN:0944-5013
1618-0623
DOI:10.1016/j.micres.2014.11.001